TGFβ和rhBMP2对人牙周膜成纤维细胞的作用  

Effects of Transforming Growth Factor and Recombinant Human Bone Morphogenetic Protein 2 on the Proliferation and Differentiation of Human Periodontal Ligament Fibroblasts

作　　者：司晓辉[1] 刘正[1] 等 

机构地区：[1]上海第二医科大学附属第九人民医院口腔内科，上海200011

出　　处：《上海口腔医学》2002年第1期53-56,共4页Shanghai Journal of Stomatology

基　　金：国家自然科学基金 (No.30 0 0 0 191) ;中国博士后科学基金(中博基 1999第 17号 );上海市博士后科学基金 (沪科 2 0 0 0第 487号 )

摘　　要：目的　研究转化生长因子 β(TGFβ)和重组人骨形成蛋白 2 (rhBMP2 )对人牙周膜成纤维细胞 (HPDLFs)增殖和分化的作用。方法　观察TGFβ和rhBMP2单独、同时和相继应用对培养的HPDLFs增殖、碱性磷酸酶 (ALP)活性、骨钙素 (OC)合成及矿化结节形成的作用。结果　TGFβ刺激HPDLFs增殖 ,对ALP活性 ,OC合成和矿化结节形成无明显影响 ;rhBMP2对HPDLFs增殖无明显作用 ,却显著提高其ALP活性 ,刺激OC合成和矿化结节形成 ;两者同时应用时对细胞增殖和分化的作用居于单独作用之间 ;先应用TGFβ对随后rhBMP2的促分化作用无明显影响 ;先应用rhBMP2再应用TGFβ对HPDLFs的分化具有显著的刺激作用。 结论　RhBMP2能刺激HPDLFs表达成骨细胞表型 ,TGFβ对BMP2诱导的细胞分化具有明显的刺激作用 。Objective To investigate the effects of Transforming growth factor β (TGFβ) and recombinant human bone morphogenetic protein 2 (rhBMP2) on the proliferation and differentiation of human periodontal ligament fibroblasts (HPDLFs).Methods To observe the individual, simultaneous and sequential effects of TGFβ and rhBMP2 on the proliferation, alkaline phosphatase (ALP) activity, osteocalcin (OC) synthesis and mineralized nodule formation of the cultured HPDLFs. Results TGFβ significantly stimulated the proliferation of HPDLFs,but had no effects on ALP activity, OC synthesis and mineralized nodule formation. RhBMP2 had no remarkable effect on the proliferation of HPDLFs, whereas significantly stimulated the ALP activity, OC synthesis and mineralized nodule formation. Co treatment with TGFβ and rhBMP2 led to intermediate effects on cell proliferation and differentiation. Pre treatment with TGFβ did not influence the subsequent rhBMP2 action on HPDLFs differentiation. Pre treatment with rhBMP2 and subsequent with TGFβ had a remarkable stimulation on HPDLFs differentiation. Conclusion RhBMP2 can stimulate the expression of osteoblastic phenotype of HPDLFs. TGFβ significantly stimulated the rhBMP2 effects on the differentiation of HPDLFs. TGFβ and BMP2 may act at different stages to promote HPDLFs differentiation towards the osteoblast phenotype.

关 键 词：转化生长因子 重组人骨形成蛋白2 人牙周膜成纤维细胞 碱性磷酸酶 骨钙素 矿化 

分 类 号：R780.2[医药卫生—口腔医学]