叠氮钠对SH-SY5Y人神经母细胞瘤细胞膜电位和胞内β-淀粉样蛋白表达的影响  

Effects of sodium azide on the membrane potential and the expression of intracellular β-amyloid in SH-SY5Y human neuroblastoma cells

作　　者：朴景华[1] 李林[1] 张丽[1] 安文林[1] 徐艳玲[1] 

机构地区：[1]首都医科大学宣武医院药理研究室北京老年病医疗研究中心,北京市100053

出　　处：《中华老年医学杂志》2002年第2期127-130,共4页Chinese Journal of Geriatrics

基　　金：北京市自然科学基金资助项目 (No .7982 0 0 6 ) ;北京市重点科技实验室项目 (No .95 1890 6 0 0 ) ;北京市科技项目 (No .95 2 6 0 180 0 );国家人事部资助优秀留学回国人员科研项目 (1998)

摘　　要：目的　观察线粒体呼吸链复合体Ⅳ抑制剂叠氮钠对SH SY5Y细胞的细胞膜电位及胞内 β 淀粉样蛋白 (Aβ)表达水平的影响 ,以探讨用叠氮钠建立模拟Alzheimer病 (AD)脑中神经细胞能量代谢障碍的细胞模型的可能性。　方法　不同浓度的叠氮钠损伤细胞后 ,用激光共聚焦显微镜观察细胞膜电位 ;用免疫细胞化学法检测细胞内 β 淀粉样蛋白前体蛋白 (APP)和Aβ的表达水平。　 结果　叠氮钠在浓度 2 5～ 10 0mmol/L时 ,与细胞共孵育 4h ,可使细胞膜电位去极化程度明显增高 ,并引起胞内Aβ表达显著增高 ,但对胞内APP表达水平影响不明显。　Objective In order to develop a new Alzheimer's disease (AD)-like cell model which mimics the disorder of energy metabolism in AD brain, the effects of sodium azide (a specific inhibitor of mitochondrial complex Ⅳ) on the membrane potential and the expression of intracellular β-amyloid(Aβ) were investigated. Methods After the SH-SY5Y cells were exposed to different concentrations of sodium azide, the membrane potential of cells was observed with fluorescent dye DiBAC4(3) by laser scanning confocal microscope, and the levels of β-amyloid precursor protein (APP) and Aβ were determined by immunocytochemistry. Results Sodium azide (25-100 mmol/L, after 4 h of incubation) depolarized the membrane potential and increased the Aβ production, but it showed no effect on the expression of APP. Conclusions The results suggest that the cell model induced by sodium azide might be one of the candidate cell models to imitate the pathological changes of AD associated with the disorder of mitochondrial complex Ⅳ.

关 键 词：膜电位 淀粉样Β蛋白 线粒体 阿尔茨海默病 神经母细胞瘤 叠氮钠 

分 类 号：R749.161[医药卫生—神经病学与精神病学]