角燕提取物抑制肝癌生长的实验研究  

A study of the inhibitory effect of Manta birostris extract on the growth of liver cancer

作　　者：沈先荣[1] 贾福星[1] 蒋定文[1] 王玲[1] 储智勇[1] 王曦[1] 雷呈祥[1] 孙加红[1] 杨晓燕[1] 

机构地区：[1]海军医学研究所海洋生物研究中心,上海200433

出　　处：《中华航海医学与高气压医学杂志》2002年第1期48-50,共3页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的　观察深海鱼类角燕提取物对肝癌的抑制作用。方法　采用盐溶液抽提 ,有机溶剂分级沉淀 ,分子筛层析等步骤 ,从海洋鱼类—角燕的药用部位获得角燕提取物。选用 QGY772 1人肝癌细胞体外实验模型 ,MTT法观察角燕提取物在体外对肝癌细胞生长的抑制作用 ;选用小鼠 HAC肝癌模型 ,评价角燕提取物在体内对肝癌生长的抑制作用。结果　角燕提取物显著抑制 QGY772 1人肝癌细胞的生长 ,且呈明显的剂量依赖关系 ,但对人肺成纤维细胞 (HL F)的生长无明显影响。角燕提取物在剂量为0 .2 5～ 1.0 0 g/kg的范围内对小鼠 HAC肝癌的生长均具有显著的抑制作用 ,并呈较好的剂量依赖关系 ;在剂量为 1.0 0 g/kg时 ,对小鼠 HAC肝癌的抑瘤率为 5 5 .5 0 %。Objective To study the effect of the extract from sea fish Manta Birostris on the growth of liver cancer.Methods The sea fish Manta sirostris was extracted using 10 mmol/L PB solution(containing 2 mol/L NaCl and 3 mmol/L EDTA),then fractionally precipitated with 40%~80% alcohol.Further purification of the extract was achieved by Sephacryl S-300 gel filtration.With the method of MTT,the effect of Manta birostris extract on the growth of human liver QGY7721 cancer cells was assayed.The mice HAC liver cancer model was used to determine the inhibitory effect of this extract on the cancer growth.Results It was shown that the Manta birostris extract inhibited the growth of QGY7721 cells significantly but with no effect on HLF cells.The extract inhibited potentially the growth of HAC liver cancer,and the inhibition rate was 55.50% in the dose of 1.00 g/kg.Conclusions The Manta birostris extract can inhibit the growth of liver cancer effectively.

关 键 词：海洋生物 角燕 肝癌 肿瘤防治 

分 类 号：R735.7[医药卫生—肿瘤] 282.74[医药卫生—临床医学]