脂质体转染胞嘧啶脱氨酶基因、放射线、树突细胞联合对直肠癌细胞杀伤作用的研究  

Three-pronged approach to treat rectal cancer: liposome transfer cytosine deaminase gene, ionizing radiation and dendritic cells

作　　者：李世拥[1] 吕文平[1] 安萍[1] 于波[1] 

机构地区：[1]北京军区总医院普通外科

出　　处：《中华外科杂志》2002年第4期274-276,共3页Chinese Journal of Surgery

基　　金：国家自然科学基金资助项目 (3 9870 73 7)

摘　　要：目的　探讨脂质体转染胞嘧啶脱氨酶 (cytosinedeaminase ,CD)基因、放射线、树突细胞(dendriticcells,DCs)三者联合治疗直肠癌的安全有效性。　方法　应用放射线促进脂质体转染CD基因 ,再利用放射的增敏作用将直肠癌细胞杀死后与DCs和T细胞共同孵育 ,通过测定3 H标记的胸腺嘧啶糖苷摄取情况 ,观察不同DCs数目对T细胞增殖的影响并用MTT法检测不同效靶比下的肿瘤细胞的杀伤率。　结果　CD与 5 氟胞嘧啶联合放射线杀死直肠癌细胞后所产生的凋亡小体和其他肿瘤抗原可有效的被DCs递呈给T细胞 ,产生细胞毒淋巴细胞 ,对肿瘤产生特异地杀伤作用。 30∶1、15∶1、1∶1效靶比的癌细胞的杀伤率分别为 98 1%、76 2 %和 37 8%。　结论　脂质体转染CD基因、放射线、DCs三者联合具有相互协同作用 。Objective To investigate the anticancer activity of three pronged approach: liposome transfer cytosine deaminase (CD) gene,ionizing radiation, and dendritic cells (DCs). Methods After rectal cancer cells were killed by CD and 5 FC and radiotherapy with improved liposome transfection and radiosensitization, the killed cancer cells,DCs and T lymphocytes were incutabtated together. The uptake of 3H TdR weighed the immune activity of DCs. The effect of anticancer was monitored by MTT. Results DCs acquired the apoptosis body and other antigen from the killed cancer cells and presented them to T lymphocytes. The induced CTL killed the cancer cells powerfully and selectively. The mortality of rectal cancer cells was 98 1%,76 2%,37 8% at the ratio of efficacy to target of 30∶1,15∶1,1∶1, respectively. Conclusion The three pronged approach may represent a powerful and safe means to selectively destroy cancer cells.

关 键 词：脂质体 基因 Γ射线 树突细胞 直肠癌 

分 类 号：R735.37[医药卫生—肿瘤] R944.9[医药卫生—临床医学]