脂质体转染AFP基因构建树突状细胞肝癌瘤苗及体外活性检测  

The construction of dendritic cell-based hepatocellular carcinoma vaccine encording AFP

作　　者：范强[1] 钟翠平[1] 陈空[1] 王国强[1] 傅继东[1] 吴超群[2] 

机构地区：[1]复旦大学医学院组织胚胎学教研室,上海200032 [2]复旦大学遗传所,上海200032

出　　处：《中国免疫学杂志》2002年第4期255-258,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(No.39770387);高校博士点基金 (No.9839)资助

摘　　要：目的：探讨以ＡＦＰ为靶点，构建ＡＦＰ－ＤＣ肝癌瘤苗及其抗肝癌免疫治疗的可能性。方法：应用脂质体将ＡＦＰ基因转染体外培养的未成熟 ＢＭＤＣ，构建 ＡＦＰ－ＤＣ肝癌瘤苗，以流式细胞术、Ｗｅｓｔｅｒｎ ｂｌｏｔ、３Ｈ－ＴｄＲ法和 ＭＴＴ法等检测其免疫活性。结果：ＡＦＰ－ＤＣ瘤苗不仅能产生和分泌ＡＦＰ，而且能上调自身的Ｂ７分子和ＭＨＣ分子，明显刺激Ｔ细胞增殖及提高ＣＴＬ的杀伤作用。结论：提示肝癌相关基因ＡＦＰ可作为抗肝癌基因治疗的切入点。Objective: To construct an AFP-DC vaccine and investigate its therapeutic function against HCC Methods: AFP was successfully transfected into cultured immature BMDC with lipofectin. Antitumor activity of the vaccine was studied in vitro by using Western blot, flow cytometry, MLR and cototoxicty assay. Results: The vaccine was confirmed capable of not only synthesizing and secreting AFP, but also up-regulating the expression of surface molecules of B7 and MI-IC. The immunization was effective in priming of specific responses and immune protection against HCC. Conclusion:The results indicated that AFP could be considered as a traget in gene immunotherapy of HCC.

关 键 词：树突状细胞 DNA疫苗 AFP 基因传染 肝癌 

分 类 号：R730.3[医药卫生—肿瘤] R735.7[医药卫生—临床医学]