杂色曲霉素对体外培养人外周血白细胞介素Ⅱ分泌影响的研究  

Effects of sterigmatocystin on interleukin-2 secretion of human peripheral blood mononuclear cells in vitro

作　　者：黄向华[1] 张祥宏[1] 严霞[1] 尹桂然[2] 谭延伟[3] 李月红[1] 刘艳丽[1] 杨建柱[1] 王俊灵[1] 王凤荣[1] 

机构地区：[1]河北医科大学基础所病理室,石家庄050017 [2]河北医大第二医院妇产科 [3]河北省血液中心

出　　处：《卫生研究》2002年第2期112-114,共3页Journal of Hygiene Research

基　　金：河北省自然科学基金资助项目 (No .30 0 342 )

摘　　要：杂色曲霉素是我国肿瘤高发区粮食中的优势污染霉菌毒素。为探讨杂色曲霉素 (ST)对人体免疫机能的影响 ,采用双抗体夹心ELISA方法对ST作用后人外周血单核细胞 (HPBMc)培养上清液中白血细胞介素Ⅱ (IL 2 )的分泌水平进行了检测。结果表明 ,不同浓度 ( 0 0 3 12 5～ 2mg L)ST处理 2 4h后 ,体外培养的HPB Mc的IL 2分泌均受到一定程度的抑制 ,其中以较低浓度ST( 0 0 3 12 5～ 0 12 5mg L)和较高浓度ST( 1～ 2mg L)抑制作用最明显 (P <0 0 5 )。在ST 1mg L作用 1～ 64h的时间范围内 ,ST对HPBMcIL 2的分泌总体表现抑制作用。ST处理后 8～ 64h ,随ST处理时间的延长 ,对IL 2分泌的抑制作用逐渐增强 (r =0 82 2 ,P <0 0 5 )。本研究结果提示 ,ST对HPBMcILSterigmatocystin (ST) is one of predominant contaminating mycotoxins in foodstuffs and grains of high incidence areas of malignant tumors in China. The effect of ST on interleukin 2 (IL 2) secretion of human peripheral blood mononuclear cells (HPBMc) in vitro was determined with enzyme linked immunosorbent assay (ELISA) method to explore its putative effects on human immune function. ELISA analysis revealed that ST treatment generally showed negative effects on the IL 2 secretion of HPBMc in vitro. As the ST concentration changes, the inhibiting effects were different. The inhibiting effects at low concentrations (0 03125～0 125mg/L) and high concentrations (1～2mg/L) were stronger than the other concentrations( P <0 05). The time effect analysis (ST 1mg/L) showed that inhibiting effects of ST on IL 2 secretion of HPBMc could be seen to a variable degree from 1 to 64 h after ST treatment, while a significant time effect correlation could be found from 8 to 64h ( r =0 822, P <0 05). The results obtained in present study showed that ST has certain inhibiting effects on the secretion of IL 2 of HPBMc in vitro.

关 键 词：杂色曲霉素 人外周血单核细胞 白细胞介素Ⅱ 肿瘤 霉菌毒素 致癌性 免疫机能 

分 类 号：R996.2[医药卫生—毒理学] R730.231.3[医药卫生—药学]