五氧化二砷体外对NB4细胞增殖及程序化死亡作用的研究  

In vitro study on effect of As_2O_5 on NB4 cells proliferation and programmed cell death

作　　者：李晶[1] 孙关林 陈惠仁[3] 

机构地区：[1]镇江医学院附属医院,212001 [2]上海市血液学研究所 [3]空军总医院

出　　处：《江苏医药》2002年第4期251-253,共3页Jiangsu Medical Journal

摘　　要：目的 探讨五氧化二砷(Ａｓ２Ｏ５)对急性早幼粒白血病(ＡＰＬ)细胞ＮＢ４增殖及细胞程序化死亡的影响。方法 用细胞生长曲线、集落培养、流式细胞仪检测、ＤＮＡ电泳、ｂｃｌ－２蛋白和ｐ５３蛋白表达等方法观察ＮＢ４细胞增殖、凋亡和癌基因表达情况。结果Ａｓ２Ｏ５能显著地抑制ＮＢ４细胞增殖和细胞生长活力,诱导ＮＢ４细胞程序化死亡,下调ｐ５３、ｂｃｌ－２蛋白表达,并能抑制造血祖细胞集落形成。结论 Ａｓ２Ｏ５对 ＡＰＬ细胞 ＮＢ４具有较强的抑制和诱导凋亡作用,可能成为治疗 ＡＰＬ有效的药物之一。Objective To study the effect of As2O5 on NB4 cell proliferation and programmed cell death. Methods NB4 cell proliferation, apoptosis and cancer gene expression were examined by cell growth curve, CFU-NB4 culture, flow cytometry, DNA electrophoresis, p53 and bcl-2 protein expression. Results As2O5 could significantly inhibit the proliferation of NB4 cell, induce programmed cell death and down-regulate p53, bcl-2 expression. Moreover, it could also inhibit CFU-GM and BFU-E in bone marrow cultures of normal human. Conclusion As2O5 could induce apoptosis and inhibit proliferation on acute promyelocytic leukemia(APL) cell line NB4. As2O5 may be one of effective medicines in the treatment of APL.

关 键 词：白血病 NB4细胞 细胞程序化死亡 五氧化二砷 细胞增殖 实验研究 

分 类 号：R733.7[医药卫生—肿瘤] R73－36[医药卫生—临床医学]