宫颈癌p53外显子7突变与HPV感染的关系  

Relationship between p53 (exon 7) mutation and HPV infection in human cervical cancers

作　　者：李惠芳[1] 常艳丽[1] 

机构地区：[1]山西省长治医学院中心实验室,046000

出　　处：《肿瘤防治研究》2002年第2期99-101,共3页Cancer Research on Prevention and Treatment

基　　金：山西省自然科学基金资助项目(940 36)

摘　　要：目的　探讨p5 3外显子 7突变与HPV1 6、1 8型感染在宫颈癌发生中的作用及相互关系。方法　采用聚合酶链反应 (PCR)、单链构象多态性 (SSCP)、限制性酶解片段长度多态性 (RFLP)分析等方法对 49例宫颈癌组织石蜡包埋标本中p5 3外显子 7的突变与HPV1 6、1 8型感染进行了检测。结果　p5 3外显子 7的突变率 1 0 .2 0 % ,HPV DNA阳性率 87.76% ,二者差异显著 (χ2 =5 5 .90 1 ,P <0 .0 0 1 ) ,突变位点多见于密码子 2 2 9(1 /5 )、2 47(2 /5 )、2 49(1 /5 ) ;HPV1 6 DNA阳性率 87.76%显著高于HPV1 8 DNA阳性率 3 8.78%(χ2 =2 3 .2 2 7,P <0 .0 0 1 ) ;p5 3外显子 7突变与HPV感染可共存于同一标本中。结论　宫颈癌p5 3外显子7突变热点是密码子 2 2 9、2 47、2 49;少数宫颈癌是由于 p5 3外显子 7突变或 p5 3外显子 7突变与高危HPV感染相互作用所致 ,大多数宫颈癌是由于高危HPV感染尤其是HPV1Objective To investigate the relationship between p53 (exon 7) mutations and human papillomavirus (HPV) 16?18 infections in human cervical cancer. Methods p53 (exon 7) mutations and HPV16?18 infections were examined by polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP) and restriction fragment length polymorphism(RFLP) analysis in the 49 cervical cancers. Results Significant difference (χ 2 =55.901, P <0.001) between p53 (exon7) mutations 5/49 (10.20%) and HPV infections 43/49 (87.76%) , and significant difference (χ 2 =23.227, P <0.001) between HPV16 infections 43/49 (87.76%) and HPV18 infections 19/49 (38.78%) were detected. p53 (exon7) mutations were in coden 229 (1/5)?247 (2/5)?249 (1/5). p53(exon7) mutations and HPV infections were simultaneously found in one cervical cancer. Conclusion p53 (exon 7) mutational hotspots existed in the coden 229?247?249. p53 (exon 7) mutations and the interaction of p53 (exon 7) mutations and HPV infections are important factor for minor part of cervical cancers, but HPV16 infection is a important factor to the major part of the cervical cancers.

关 键 词：抑癌基因 P53 HPV 基因突变 宫颈癌 HPV感染 

分 类 号：R737.33[医药卫生—肿瘤]