重组腺病毒介导的人野生型p53、GM-CSF和B7-1基因在肝癌细胞中的表达  

Wild-type p53,GM-CSF,B7-1 genes transduced with recombinant adenovirus expression in hepatoma cell lines in vitro

作　　者：施明[1] 王福生[1] 刘明旭[1] 金磊[1] 雷周云[1] 邱兆华[2] 高兰兴[3] 吴祖泽[2] 

机构地区：[1]解放军302医院生物工程研究室,北京100039 [2]军事医学科学院放射医学研究所 [3]军事医学科学院卫生学环境医学研究所

出　　处：《肿瘤防治研究》2002年第2期123-125,共3页Cancer Research on Prevention and Treatment

摘　　要：目的　观察腺病毒载体对肝癌细胞的转染效率及其介导的人野生型 p5 3、GM CSF和B7 1基因在肝癌细胞中的表达。方法　不同MOI的Ad GFP感染肝癌细胞 ,48h后计算感染效率 ;BB 1 0 2以5 0MOI感染肝癌细胞 ,48h后免疫组化法及westernblot检测 p5 3表达 ,ELISA检测GM CSF的含量 ,FACS测定B7 1的表达。结果　MOI为 5 0 pfu/细胞时对肝癌细胞的转染效率达 80 %以上 ,目的基因均可在肝癌细胞中高效表达。结论　腺病毒载体对肝癌细胞具有较高的转染效率 ,目的基因均可在肝癌细胞中高效表达 ,为进一步研究BB 1 0Objective To evaluate the transferring efficiency of recombinant adenoviral vector(BB\|102) expressing the human wild type p53,GM\|CSF and B7\|1 genes into hepatocellular carcinoma(HCC) cell lines in vitro. Methods HCC cell lines were transferred with mock adenoviral vector expressing green fluorescent protein(Ad\|GFP) at a series concentrations of MOI. p53, GM\|CSF, and B7\|1 expressing in HCC cells were determined by western blot, ELISA method and flow\|cytometric analyses respectively. Results When 50MOI of recombinant adenoviral vectors was used, over 80% of target tumor cells could be efficiently transferred. High levels of p53, GM\|CSF, and B7\|1 expressed in HCC cells transferred with BB\|102.Conclusion Adenoviral vectors expressing human wild type p53, GM\|CSF and B7\|1 genes could be used as an efficient vector to expressing gene \|in\|interest in HCC cell lines. Our study provides a experimental evidence that the BB \|102 might be applicable in clinical gene therapy against HCC.

关 键 词：重组腺病毒 肝癌细胞 p53 GM-CSF B7-1 基因表达 

分 类 号：R735.7[医药卫生—肿瘤]