奥曲肽和表皮生长因子受体单克隆抗体诱导人胃癌细胞凋亡  

Apoptosis Induced by Gut Peptide Hormone Long Acting Somatostatin Analogue and Epidermal Growth Factor Receptor Monoclonal Antibody in Human Gastric Carcinoma Cell

作　　者：陈穗[1] 范平[1] 齐凤[2] 尹格平[2] 李定国[3] 

机构地区：[1]解放军第113医院传染科,315040 [2]济南军区总医院消化内科 [3]上海第二医科大学附属新华医院消化内科

出　　处：《胃肠病学》2002年第2期90-90,118,共2页Chinese Journal of Gastroenterology

摘　　要：目的：定量检测奥曲肽（ＳＭＳ）和表皮生长因子受体单克隆抗体（ＥＧＦＲＭＣＡｂ）对人胃癌细胞系 ＳＧＣ－７９０１的诱 导凋亡作用。方法：将不同浓度的ＳＭＳ和ＥＧＦＲＭｃＡｂ与胃癌细胞共同孵育，用流式细胞术分析其细胞凋亡情况。 结果：ＳＭＳ ５ × １０－５和ＥＧＦＲＭｃＡｂ１：１００与胃癌细胞共同孵育７２ ｈ，胃癌细胞凋亡率分别为７．９６％和７．４９％，与对 照组（１．１９％湘比，有显著差异ｐ＜０．０５）。结论：诱导细胞凋亡是胃肠肽类激素发挥其抗肿瘤作用的重要途径之一。Aims: To examine the apoptostic effects in human gastic carcinoma cell line SGC-7901 induced by long acting somatostatin analogue(SMS) and epidermal growth factor receptor monoclonal antibody(EGFRMcAb). Methods: SGC-7901 cells were incubated with SMS or EGFRMcAb in different concentrations, and then analyzed by flow eytometry. Results: While SGC-7901 cells were treated by SMS 5 ×10-5or EGFRMcAb 1:100 for 72 hours, the percentage of apoptosis was 7.96% and 7.49%(P< 0.05) respectively. Conclusions: Apoptosis mechanism is one of the important ways for anti-tumour effects of gut peptide hormones.

关 键 词：奥曲肽 脱噬作用 流式细胞术 表皮生长因子-尿抑胃素受体 单克隆抗体 抗肿瘤作用 胃肿瘤 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]