重金属污染土壤毒性的斜生栅藻生长抑制实验诊断  

Toxicity assessment of soil contaminated by heavy metals using algae growth inhibition test.

作　　者：李彬[1] 李培军[2] 王晶[2] 张海荣[2] 杨桂芬[2] 徐大伟[1] 苏宝玲[1] 

机构地区：[1]沈阳大学生命与环境学院,沈阳110016 [2]中国科学院沈阳应用生态研究所,沈阳110016

出　　处：《应用生态学报》2002年第3期331-334,共4页Chinese Journal of Applied Ecology

基　　金：国家重点基础研究发展规划项目(G19990 1180 8 5 );中国科学院知识创新重要方向资助项目 (KZCS2 SW 416)

摘　　要：应用斜生栅藻 (Scenedesmusobliquus)对重金属污染土壤的毒性进行诊断 ,结果表明 ,斜生栅藻的增长率与土壤中的重金属含量明显相关 ,并且随重金属投加量的增加 ,其增长率逐渐降低 .对两种测试参数进行比较 ,发现采用细胞数增长率作为土壤毒性的检测指标要比采用光密度增长率更为敏感 .当采用细胞数增长率作为检测指标时 ,4种重金属的EC50 顺序为 :Pb >Cu >Zn >Cd ;采用光密度增长率作为检测指标时 ,4种重金属的EC50 顺序为 :Pb >Cu >Cd >Zn .复合污染情况下 ,由于金属间的协同作用 ,使土壤的毒性比单一重金属污染时有明显增强 。Scenedesmus obliquus growth inhibiton test was carried out to assess the toxicity of soil contaminated by heavy metals. The results showed that the growth rate of Scenedesmus obliquus was positively related to the concentration of heavy methals added to soil,and it was decreased with the increasing concentration of heavy metals. Two parameters were chosen and the sensivities were compared.It was found that the cell growth rate was more sensitive than OD growth rate. The orders of general detection limit by EC\-\{50\} of different metals were Pb>Cu>Zn>Cd by using cell growth rate as the test index and Pb>Cu>Cd>Zn by using OD as the test index. This study also indicated that under the complex pollution condition,the toxicity of heavy metals in soil was stronger than that in the case of soil contaminated by single metal.

关 键 词：生长抑制实验 土壤污染 毒性 斜生栅藻 重金属污染 生态毒理指标 诊断 

分 类 号：X131.3[环境科学与工程—环境科学]