Relationship Between Ca^(2+) and Plant Ethylene Response  

钙与植物乙烯反应的关系研究(英文)

作　　者：张艳萍[1] 朱本忠[1] 罗云波[1] 

机构地区：[1]中国农业大学食品学院,北京100094

出　　处：《Acta Botanica Sinica》2002年第4期422-426,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家重点基础研究规划发展项目 (G19990 1170 1);国家杰出青年基金 ( 3982 5 118)~~

摘　　要：The relationship between Ca 2+ and ethylene response was investigated through analyzing the effect of Ca 2+ on the response to ethylene in etiolated tomato (Lycopersicon esculentum Mill cv. Lichun) seedling grown in darkness. When the etiolated tomato seedlings were treated with different concentrations of Ca 2+, the 'triple response' phenotype, ethylene production, the expression of ethylene receptor gene NEVER-RIPE (NR) and the content of cytosolic CaM were determined. With the concentration of Ca 2+ in the culture medium increasing from 0 mmol/L to 3.8 mmol/L, the 'triple response' phenotype of etiolated tomato seedling was correspondingly strengthened; meanwhile the ethylene production, the amount of NR gene expression and the concentration of CaM increased respectively. However, when the concentration of Ca 2+ was increased from 3.8 mmol/L to 10 mmol/L, the phenotype of 'triple response', ethylene production, NR gene expression, and the CaM content didn't increase further, but decreased consequently. The results indicated that the effect of Ca 2+ on the ethylene triple response in etiolated tomato seedling was relevant to ethylene biosynthesis and ethylene receptor gene expression which were influenced by applied Ca 2+, and these effects might be mediated through the change of CaM concentration in plant cell.研究了Ca2 + 对番茄 (LycopersiconesculentumMillcv.Lichun)黄化幼苗乙烯反应的影响。通过测定不同Ca2 + 浓度条件下番茄黄化幼苗的“三重反应”、内源乙烯释放量、乙烯受体基因NEVER_RIPE(NR)表达量及胞内CaM含量的变化 ,结果发现 ,随着培养基中Ca2 + 浓度从 0mmol/L增加到 3.8mmol/L ,番茄黄化幼苗的“三重反应”表型明显增强 ,内源乙烯释放量、NR基因的表达量及胞内CaM的含量都有不同程度的增加 ;当Ca2 + 浓度由 3.8mmol/L进一步增加到 10mmol/L时 ,番茄黄化幼苗“三重反应”表型受到抑制 ,内源乙烯释放量、NR基因的表达量及胞内CaM的含量都有所下降。因此 ,Ca2 + 对番茄黄化幼苗“三重反应”的影响与Ca2 + 调节内源乙烯合成和乙烯受体基因的表达有关 ,而且Ca2 +

关 键 词：Ca    2+ triple response ethylene signal transduction CAM 

分 类 号：Q945[生物学—植物学]