广西眼镜蛇毒细胞毒素的分离及对人鼻咽癌等细胞抑制作用的研究  

Studies of cytotoxin from guangxi cobra venom on its purification and cytotoxic effects on the nasopharyngeal and other cancer cells

作　　者：雷丹青[1] 王秋雁[1] 刘绵林[1] 舒雨雁[1] 汤圣希[1] 

机构地区：[1]广西医科大学蛇毒研究所,南宁530021

出　　处：《中国药理学通报》2002年第1期61-64,共4页Chinese Pharmacological Bulletin

基　　金：广西壮族自治区青年科学基金资助课题 ;No 9912 0 2 5

摘　　要：目的　探讨广西眼镜蛇毒细胞毒素对人鼻咽癌细胞(CNE)等多种癌细胞株的抑制作用。方法　经SephadexG 5 0 ,CM SepharoseCL 6B层析法从广西眼镜蛇毒中分离纯化获得细胞毒素 (CTX) ,采用MTT检测CTX对癌细胞株体外培养抑制作用 ,探索量效时效关系。结果　从广西眼镜蛇毒中分离到一种细胞毒素单一组分 (CTXCM 5 )对体外培养的人鼻咽癌细胞株 (CNE)、淋巴瘤细胞株 (YAC)、人宫颈癌细胞株 (HELA)和卵巢癌细胞株 (Ho8990 )的抑制有明显的剂量依赖关系 ,作用 48h的IC50 分别为 1 84,0 75 ,1 84和 2 5 9mg·L-1;随作用时间的延长 ,CTXCM 5对CNE细胞株作用最为明显 ,作用 3h和 2 4h的IC50 分别为 4 78和1 0 4mg·L-1。结论　CTXCM 5对体外培养的癌细胞株有较强的抑制作用。AIM To study the inhibitory effect of the cytotoxin (CTX) from guangxi cobra venom on human nasopharyngeal cancer cell (CNE) and other tumons cells. METHODS The cytotoxin was isolated and purified from Guangxi cobra venom by successive chromatography on Sephadex G-50 and CM Sepharose CL-6B columns. Its cytotoxic effects and does-effect relationship on human tumors cell lines were examined by MTT assay. RESULTS The inhibitory effects of CTX CM-5 on CNE, human ovarian carcinoma cell (Ho8990), uterus cervical carcinoma cell (HELA) and lymphoma (YAC) cell lines showed a definite does-effect relationship. The IC 50 (48 h in cubation) was 1.84, 2.59, 1.84 and 0.75 mg·L -1 respctively. The inhibitory effects of CTX CM-5 on CNE increased with time. The IC 50 (3 h and 24 h cubation) was 4.78 and 1.04 mg·L -1 respctively. CONCLUSION CTX from Guangxi cobra venom exhibites strong suppressive effect on cultured tumor cells line in vitro.

关 键 词：细胞毒素 鼻咽癌 MTT 眼镜蛇毒 抑癌作用 研究 

分 类 号：R739.63[医药卫生—肿瘤] R73－36[医药卫生—临床医学]