褪黑素对人胃腺癌SGC-7901细胞的抑制作用  

Inhibitory effect of melatonin on human gastric gland carcinoma SGC-7901 line

作　　者：白艳红[1] 赵晏[2] 蔡晓宏[3] 慕慧[1] 郭媛[2] 

机构地区：[1]西安交通大学理学院化学系,西安710061 [2]西安交通大学生理学教研室,西安710061 [3]西安近代化学研究所,西安710065

出　　处：《西安医科大学学报》2002年第2期144-146,共3页Journal of Xi'an Medical University(Chinese)

摘　　要：目的　研究褪黑素对人胃腺癌SGC 790 1细胞的生长抑制作用及其作用机理。方法　采用不同浓度的褪黑素通过体外细胞培养、MTT显色技术研究其抑制作用 ,通过流式细胞仪和透射电子显微镜技术观察 ,对其作用机理进行探讨。结果　褪黑素对人胃腺癌SGC 790 1细胞具有抑制作用 ,其IC30 、IG50 分别为 0 83和 1 70mmol·L- 1 。以IC30 的褪黑素处理SGC 790 1细胞 ,使其倍增时间由 31 2h延长到 38 4h。流式细胞仪观察到褪黑素导致SGC 790 1细胞G0 /G1 期比例升高 ,S期比例降低。透射电镜可观察到以IC30 的褪黑素处理的细胞 ,其超微结构发生了退行性改变。结论　褪黑素对人胃腺癌SGC 790 1细胞具有抑制作用 ,其作用机理可能是褪黑素导致细胞G0 /G1Objective To study the inhibitory effects and the mechanism of melatonin on the growth of human gastric gland carcinoma SGC 7901. Methods The inhibitory effects of melatonin with various concentrations on the human gastric gland carcinoma SGC 7901 lines in vitro were determined with MTT Method. The mechanism of the inhibition was observed by flow cytometry(FCM) and transmission electron microscopy(TEM). Results The IC 30 50 value were 0.83 mmol·L -1 and 1.70 mmol·L -1 , respectively. The population doubling time of SGC 7901 cells treated with melatonin at 0.83 mmol·L -1 was prolonged from 31.2 h to 38.4 h, as compared with that of the control group. Under FCM we found that the cell was inhibited by melatonin in G 0/G 1 phase and the cell percentage in S stage decreased. The degenerated ultra structure of the cell treated with melatonin was also observed by TEM. Conclusion The results suggest that melatonin can inhibit the growth of human gastric gland carcinoma SGC 7901 cells. The mechanism of inhibition may be that the G 0/G 1 stage of the cell cycle is delayed by melatonin.

关 键 词：褪黑素 人胃腺癌 SGC-7901细胞 抑制作用 群体倍增时间 

分 类 号：R735.2[医药卫生—肿瘤]