益气养阴方诱导急性白血病细胞凋亡的实验研究  

Experiment Study of Yiqi YangYin Formula Induces Apoptosis in Acute Leukemia Model in Mice

作　　者：孙静[1] 李震[1] 王莉[1] 臧运华[1] 张丹[1] 李军山[1] 李兰[1] 刘黎青[1] 高苏缇[1] 简隆磊[1] 

机构地区：[1]山东中医药大学,山东济南250014

出　　处：《肿瘤防治杂志》2002年第1期46-49,共4页China Journal of Cancer Prevention and Treatment

基　　金：山东省自然科学基金 (Y2 0 0 0C0 3)

摘　　要：目的 :观察益气养阴方诱导急性白血病模型小鼠细胞凋亡的作用 ,探讨益气养阴方治疗急性白血病的作用机制。方法 :以L72 12 白血病小鼠为研究对象 ,用益气养阴方浓缩液给小鼠灌胃 8d后处死 ,利用光学显微镜、DNA琼脂糖凝胶电泳等手段观察益气养阴方对急性白血病的作用。结果 :形态学观察可见细胞凋亡特征 ,DNA琼脂糖凝胶电泳图谱显示细胞凋亡现象。结论Objective To observe the effect of inducing apoptosis in acute leukemia mice by Yiqi Yanyin formula and investigate the mechanism of Yiqi Yanyin formula in treatment of acute leukemia.Methods L 7212 leukemia mice were filled with the concentrated liquid of Yiqi Yanyin formula in the stomach.After eight days,the mice were sacrified then used for in vitro studied.The effect of Yiqi Yanyin formula on acute leukemia mice was studied by suing optical microscope and DNA agarose gel electrophoresis,etc.Results The typical feature of apoptosis induced by chemotherapeutic drugs was observed by morphological examination.The phenomenon of apoptosis could be detected in DAN fragmentation electrophoregram.Conclusion This implies that inducing apoptosis in leukemia cell may play important role of morphology observation of Yiqi Yanyin formula in the treatment of acute leukemia.

关 键 词：中医药疗法 益气养阴方 急性白血病 细胞凋亡 实验研究 

分 类 号：R73-36[医药卫生—肿瘤]