口腔粘膜癌前病变及口腔鳞状上皮细胞癌p53、p16蛋白和PCNA的表达  

Study on the expression of p53、 p16 protein and PCNA in oral precancerous lesion and oral squamous cell carcinoma

作　　者：钟良军[1] 袁芳[2] 张薇[3] 

机构地区：[1]新疆医科大学第一附属医院口腔科,新疆乌鲁木齐830054 [2]新疆乌鲁木齐市口腔医院,新疆乌鲁木齐830002 [3]新疆医科大学第一附属医院病理科,新疆乌鲁木齐830054

出　　处：《新疆医科大学学报》2002年第1期12-14,共3页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区卫生厅青年医学科学研究基金 (新卫科青字 980 0 3号 )

摘　　要：目的:探讨口腔粘膜癌前病变及口腔鳞状细胞癌发生与发展过程中 ,p5 3蛋白、p16蛋白和 PCNA的表达及意义。方法:应用免疫组织化学方法对 2 0例口腔扁平苔藓、2 0例口腔白斑、2 0例口腔鳞状细胞癌分别进行检测。结果:(1)口腔鳞状细胞癌组织中突变型 p5 3蛋白阳性率为 90 .0 %,明显高于口腔白斑 (10 .0 %)和口腔扁平苔藓 (0 .0 %) (P <0 .0 0 5 )。(2 )口腔鳞状上皮细胞癌组织中 p16蛋白阳性率为 35 .0 %,明显低于口腔白斑 (70 .0 %)和口腔扁平苔藓 (90 .0 %) (P <0 .0 0 5 )。 (3) PCNA的阳性率在口腔鳞状细胞癌组织中为 90 .0 %,明显高于口腔白斑(10 .0 %)和口腔扁平苔藓 (0 .0 %) (P <0 .0 0 5 )。 结论:p5 3基因突变使 p5 3蛋白过度表达、p16蛋白表达缺失和PCNA的过度表达在口腔鳞状上皮细胞癌的发生与发展过程中起着十分重要的作用。Objective: To investigate the relationship of expression between p53、p16 protein and PCNA and their significance during the development and progression of oral precancerous lesion, and squamous cell carcinoma. Methods: Expression of p53、p16 protein and PCNA were detected in oral lichen planus (OLP) 20 cases, oral leukoplakia (OL) 20 cases and oral squamous cell carcinoma (OSCC) 20 cases by immunohistochemical techniques. Results: (1) The positive rate of p53 protein was higher in OSCC (90.0%) than in OL (10.0%) and OLP (0.0%) (P<0.005), (2) The positve rate of p16 protein was lower in OSCC (35.0%) than in OL (70.0%) and OLP (90.0%) (P<0.005). (3) The positive rate of PCNA was higher in OSCC (90.0%) than in OL(10.0%) and OLP (0.0%) (P<0.005) . Conclusion: p53 gene mutation resulting in over-expresion of p53 protein, the low-expression of p16 protein and over-expression of PCMA may play important roles in oral squamous cell carcinoma development and progression.

关 键 词：P53蛋白 P16蛋白 PCNA 口腔粘膜癌前病变 口腔鳞状上皮细胞癌 

分 类 号：R739.850.2[医药卫生—肿瘤] R730.213.3[医药卫生—临床医学]