Wistar大鼠插线法局灶性脑缺血/再灌注模型的实验研究  

An experimental study on focal cerebral ischemia/reperfusion model in Wistar rats with suture method

作　　者：罗勇[1] 董为伟[1] 

机构地区：[1]重庆医科大学临床学院神经内科,重庆400016

出　　处：《重庆医科大学学报》2002年第1期1-4,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金项目(30040013);国家教育部<高等学校骨干教师资助计划>项目

摘　　要：目的：局灶性脑缺血／再灌注模型是研究缺血性脑血管病的重要模型。本文介绍一种简便易行的插线法Ｗｉｓｔａｒ　大鼠局灶性脑缺血／再灌注模型的制备方法，并通过神经病学评分、ＴＴＣ染色、病理形态学变化的观察，对该模型的可靠性进行评价。方法：用头端处理过的尼龙钓丝，从颈外动脉向颈内动脉插入，可逆性阻断大鼠一侧大脑中动脉（ＭＣＡ）。结果：大鼠ＭＣＡ阻断后１．５ｈ，同时出现Ｈｏｒｎｅｒ＇ｓ征阳性、提尾悬空时梗塞对侧前肢屈曲、内收、自主运动时身体向偏瘫侧划圈的动物，ＴＴＣ染色能清楚地显示梗塞范围，且相对较稳定，光镜下可见脑缺血后的病理改变。阻断后３ｈ及再灌注３、６ｈ时的病理改变加重，神经病学评分同前。结论：该改良法制备的模型简便易行、稳定性好、结果可靠，是用于研究局灶性脑缺血／再灌注的较为理想的实验动物模型。Objective: Focal cerebral ischemia/reperfusion model is an important model in studying ischemic cerebrovascular diseases. In this paper we introduce a simple suture method to produce the focal cerebral ischemia/reperfusion, and evaluate the reliability of this model in Wistar rats with the degree of neurologic impairment,TTC stain, histologic changes. Methods: Nylon thread was inserted into the internal carotid artery in Wistar rat to achieve an animal model of reversible middle cerebral artery occlusion(MCAO). Results: 1.5h after MCAO, the rat displays Horner's sign, forelimb flexion, circling behavior. TTC stain clearly indicates the infarction size and relative stability, and can observe histological changes of ischemia. 3h after MCAO and 3h, 6hr after reperfusion, the ischemic changes become heavier, but there is no change observed in the score of neurologic behavior. Conclusion: This improved model is simple, stable and reliable. It is advisable to use this experimental animal model to study focal cerebral ischemia/reperfusion.

关 键 词：局灶性脑缺血 再灌注 插线法 动物模型 WISTAR大鼠 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学] R－332[医药卫生—临床医学]