鼻咽癌患者血T淋巴细胞核仁形成区嗜银蛋白检测的价值  

Detection of silver-stained nucleolar organizer regions in the peripheral blood T lymphocytes in nasopharyngeal carcinoma patients

作　　者：李先明[1] 吴冬[1] 陈善义[1] 任浙平[1] 陈亦欣[1] 王秀清[1] 

机构地区：[1]暨南大学医学院第二附属医院肿瘤放射治疗科,深圳518020

出　　处：《中华放射肿瘤学杂志》2002年第1期39-41,共3页Chinese Journal of Radiation Oncology

摘　　要：目的　探讨外周血T淋巴细胞核仁形成区嗜银蛋白 (Ag NORs)的检测在鼻咽癌诊治中的临床价值。方法　对 36例正常人、73例初治、11例复发和 (或 )转移及 32例放射治疗后随诊鼻咽癌患者进行外周血T淋巴细胞Ag NORs检测 ,并对部分初治患者放射治疗前、期间及后的Ag NORs含量进行动态观察 ,结果以核仁银染面积与细胞核面积的比值 (I.S % )表达。结果　各组鼻咽癌患者外周血T淋巴细胞Ag NORs含量均明显低于正常人 (P <0 .0 1) ,复发与初治和随诊患者间也有显著差异(P <0 .0 1)。Ⅲ、Ⅳ期患者Ag NORs含量低于Ⅰ、Ⅱ期患者 ,差异无显著性 (P >0 .0 5 )。放射治疗期间Ag NORs含量较放射治疗前明显降低 (P <0 .0 1) ,放射治疗后与放射治疗前比较无显著差异 (P >0 .0 5 )。结论　外周血T淋巴细胞Ag NORs检测在鼻咽癌患者的疗效观察。Objective To evaluate the clinical significance of detecting silver stained nucleolar organizer regions (Ag NOR) in the peripheral blood T lymphocytes in nasopharyngeal carcinoma (NPC) patients.Methods Ag NOR in the peripheral blood T lymphocytes detected in 36 healthy subjects served as control. Those in 73 newly diagnosed but untreated, 11 recurrent ( and / or metastatic ) and 32 treated NPC patients in follow up were monitored. The dynamic variations in the level of Ag NORs in the peripheral blood T lymphocytes in the pre radiotherapy(pre RT), during RT and post RT were evaluated in part of the newly diagnosed patients. Results The level of Ag NORs in the peripheral blood T lymphocytes in all groups of NPC patients were significantly lower as compared to the health controls(P<0.01). There were significant differences in the level of Ag NORs between recurrent and newly diagnosed patients and between recurrent patients and patients in their follow up(P<0.01), with the recurrent patients having the lowest levels. The level of Ag NORs of stage Ⅲ and Ⅳ patients was lower than that of stage ⅠandⅡ patients, though without any significant difference (P>0.05). The level of Ag NORs during RT significantly decreased as compared to that of pre RT(P<0.01). No significant difference in the level of Ag NORs between pre RT and post RT was found (P>0.05). Conclusions Detection of Ag NORs in the peripheral blood T lymphocytes is of significance in evaluating the outcome, predicting prognosis and even in making the diagnosis and staging for NPC patients.

关 键 词：鼻咽肿瘤 放射治疗 T淋巴细胞 核仁形成区嗜银蛋白 AG-NORS 

分 类 号：R739.63[医药卫生—肿瘤]