食管癌、胃癌细胞对氧化砷促凋亡的敏感性与固有活性氧水平有关  

The sensitivity of the gastric and esophageal carcinoma cells to arsenic trioxide is associated with the inherent cellular level of reactive oxygen species

作　　者：高飞[1] 易静[1] 史桂英[1] 李慧[1] 石学耕[1] 汤雪明[1] 

机构地区：[1]上海第二医科大学细胞生物学教研室,200025

出　　处：《中华消化杂志》2002年第2期70-72,共3页Chinese Journal of Digestion

基　　金：国家自然科学基金 (3 973 0 2 70 );上海市教委高校科技发展基金 (2 0 0 0B10 1)

摘　　要：目的　探讨食管癌、胃癌细胞的固有活性氧 (reactiveoxygenspecies,ROS)水平与细胞对三氧化二砷 (As2 O3 )促凋亡敏感性之间的关系。方法　用小剂量 (2 μmol/L)As2 O3 作用于人胃癌细胞和食管癌细胞株各一对 (SGC790 1、MKN4 5和EC/CUHK1、EC186 7) ,证实As2 O3 促凋亡敏感性在SGC790 1和MKN4 5之间及EC/CUHK1和EC186 7之间均有差异。然后在不加As2 O3 的情况下 ,用活性氧捕获剂双氢罗丹明 12 3(DHR 12 3)孵育细胞 (DHR 12 3在细胞内被ROS氧化为发出荧光的罗丹明 12 3) ,通过流式细胞仪检测细胞内罗丹明 12 3的荧光而测得细胞内固有ROS水平。结果　对As2 O3 敏感的SGC790 1细胞的固有活性氧水平比As2 O3 不敏感的MKN4 5细胞高 ,对As2 O3 敏感的EC/CUHK1细胞的固有活性氧水平比As2 O3 不敏感的EC186 7细胞高。结论　食管癌、胃癌细胞固有ROS水平的差异与细胞对As2 O3Objective To explore the association of the inherent cellular level of reactive oxygen species (ROS) with the sensitivity of the gastric and esophageal carcinoma cells to arsenic trioxide (As 2O 3). Methods The difference of sensitivity to apoptosis induction by low concentration (2 μmol/L) of As 2O 3 between the gastric carcinoma cell line SGC7901 and MKN45, and between the esophageal carcinoma cell line EC/CUHK1 and EC1867 was firstly demonstrated. SGC7901 was more sensitive than MKN45, and EC/CUHK1 more sensitive than EC1867 to As 2O 3. Then dihydrogenrhodamine 123 (DHR123), as a ROS capture, was incubated with the cells in the absence of As 2O 3. The fluorescent intensity of rhodamine 123, the product of cellular oxidation of DHR 123, was assayed by flow cytometry. The ROS levels was thus detected. Results Inherent cellular ROS level is higher in SGC7901 and EC/CUHK1. They are more sensitive to As 2O 3, than the corresponding cell line MKN45 and EC1867. Conclusions The difference in inherent cellular ROS level of gastric and esophageal carcinoma cells is associated with the cellular sensitivity to apoptosis induced by As2O 3.

关 键 词：食管癌 胃癌 氧化砷 促凋亡 敏感性 固有活性氧 三氧化二砷 细胞凋亡 治疗 

分 类 号：R735[医药卫生—肿瘤]