CD95在胰腺癌细胞化疗敏感性中的作用研究  

Effect of CD95 system in the pancreatic cancer cells sensitivity to chemotherapy

作　　者：徐克强[1] 袁世珍[2] 

机构地区：[1]解放军第一五七医院消化科,广州510510 [2]中山医科大学孙逸仙纪念医院消化科

出　　处：《中华消化杂志》2002年第2期96-99,共4页Chinese Journal of Digestion

基　　金：广东省自然科学基金部分资助项目 (0 0 13 5 5 )

摘　　要：目的　探讨CD95系统在胰腺癌细胞对化疗敏感性中的作用。方法　通过脂质体介导将CD95基因转入胰腺癌细胞株SW 1990 ,G4 18筛选转导CD95基因的细胞克隆 ,Northern blot和Western blot检测转导细胞中CD95的表达 ;MTT法观察转导细胞对氟尿嘧啶 (5 FU)、阿霉素 (ADM )、双氟脱氧胞苷 (GCB)及与抗CD95单克隆抗体联合治疗的反应 ;流式细胞仪 (FCM)检测化疗药诱导转导细胞凋亡的作用。结果　CD95基因转导的SW 1990细胞可稳定地过表达CD95 ,其mRNA与蛋白的表达均较未转导及空白对照组显著增高 ,抗CD95单抗可阻滞转导细胞的生长。临床相关药物浓度的化疗药对转导细胞的生长抑制作用明显高于未转导细胞 ,加入抗CD95单抗可显著增强化疗药的作用。ADM诱导胰腺癌细胞凋亡作用较明显 ,且转导细胞显著高于未转导细胞。结论　转导CD95基因可提高SW1990细胞对化疗药的敏感性 ,部分逆转其耐药性。Objective To explore the role of CD95 system in chemotherapeutic sensitivity of pancreatic cancer cells, in an attempt to enhance the efficacy of chemotherapy by transfection CD95 gene, and to provide the evidence for the immunological treatment of pancreatic cancer. Methods CD95 gene was transfected into the pancreatic cancer cell line SW1990 by lipofectamine. The transfected cells were selected by G418. CD95 expressions of the transfected cells were detected by Northern blot and Western blot. MTT assay was used to analyze the response of the transfected cells to 5 fluorouracil, adriamycin (ADM), gemcitabine and in combination with anti CD95 monoclonal antibody (mAb). The drug induced apoptosis of transfected cells was measured by flow cytometry. Results The transfected pancreatic cancer cell SW1990 could overexpress CD95 stably. The CD95 mRNA and protein expressions were significantly increased in the transfected cells than in the controls. Anti CD95 mAb could inhibit the growth of the transfected cells. In addition, transfected cells were more sensitive to clinically relevant concentrations of chemotherapeutic drugs than non transfected cells. Anti CD95 mAb addition could enhance the cytotoxic effect of chemotherapeutic drugs. Drug induced apoptosis in ADM treated transfected cells more pronounced than in non transfected cells. Conclusions CD95 transfection could increase the sensitivity of pancreatic cancer cell SW1990 to, and partly reverse the resistance to, chemotherapeutic drugs. The combination of chemotherapeutic drugs with anti CD95 mAb showed a synergistic cytotoxicity to pancreatic cancer cells.

关 键 词：胰腺癌 药物疗法 CD95 胰腺癌 细胞 化疗敏感性 作用研究 

分 类 号：R735.9[医药卫生—肿瘤] R730.53[医药卫生—临床医学]