缺氧对视网膜Mller细胞超微结构及GFAP表达的影响  

Effects of hypoxia on the cultured Müller cells: Ultrastructure changes and GFAP expression

作　　者：苟琳[1] 张作明[1] 郭守一[1] 张萍[2] 李莉[1] 郭群[1] 

机构地区：[1]第四军医大学航空航天医学系航空临床医学教研室,陕西西安710033 [2]第四军医大学全军神经科学研究所,陕西西安710033

出　　处：《第四军医大学学报》2002年第5期472-475,共4页Journal of the Fourth Military Medical University

摘　　要：目的　探讨持续低流量缺氧条件下视网膜 Müller细胞超微结构的改变及缺氧对神经胶质酸性蛋白 (GFAP)表达的影响 .方法　采用透射电镜、western blot方法对缺氧条件下培养的视网膜 Müller细胞进行观察 .结果　缺氧 4 8h后 ,视网膜 Müller细胞的超微结构发生改变 ,细胞内 GFAP表达较对照组增高 .结论　持续低流量缺氧 4 8h。AIM To study the effects of hypoxia on the ultrastructure and GFAP's expression of Müller cells. METHODS Transmission electron microscope and western blot technique were used to observe the Müller cells cultured at hypoxia condition. RESULTS After 48 h of hypoxia, the retinal Müller cells' ultrastructure apparently changed and the glial fibrillary acidic protein (GFAP) had an increased expression compared to the control group. CONCLUSION Certain lesion would happen to the retinal Müller cells when they were insulted by 48 h hypoxia.

关 键 词：低氧 MÜLLER细胞 超微结构 神经胶质酸性蛋白 CFAP 视网膜疾病 

分 类 号：R774[医药卫生—眼科]