HPLC法测定蒙药哈斯-哈图古日-15中没食子酸的含量  

Determination the Content of Gallic Acid in Haas-Hatuguri-15 by HPLC

作　　者：周风琴[1] 赵玉英 张良[1] 姚立伟[1] 王海燕[1] 

机构地区：[1]内蒙古民族大学化学化工学院,内蒙古通辽028043 [2]内蒙古通辽天然产物开发与废弃物利用研究所,内蒙古通辽028043

出　　处：《内蒙古民族大学学报（自然科学版）》2014年第3期277-279,共3页Journal of Inner Mongolia Minzu University：Natural Sciences

基　　金：国家自然科学基金资助项目(21261015)

摘　　要：建立用HPLC法测定蒙药哈斯-哈图古日-15中没食子酸的方法.色谱柱为：Hypcrsil C18 ODS（4.6mmX250mm,5μL）,流动相为乙腈-水-磷酸（体积比为5：95：0.02），流速为0.8 mL·min-1,检测波长为273nm，柱温为30℃.没食子酸在（0.40～1.2）mg·mL-1范围内线性关系良好（y=9E＋06x-127588，r=0.9999），平均回收率94.95%（RSD=2.24%）.该方法可作为蒙药及蒙药材的没食子酸的含量测定方法.To establish a HPLC method for determination the content of Gallic Acid in Haas-Hatuguri-15. The high performance liquid chromatography（HPLC）separation was performend on Hypcrsil C18 ODS（4.6mmX250mm,5μL）. The mobile phase consisted of acetonitrile-water-phosphate （The volume ratio of 5：95：0.02 ）. The flow rate was 0.8mL·min-1 , the detection wavelength was 273nm, the column temperation was 30℃. Gallic Acid showed a good lin-ear relationship（y=9E＋06x-127588,r=0.9999）in the range of 0.40-1.20mg·mL-1 , the average recovery rate was 94.95%, RSD=2.24%. This method can be used as the Gallic Acid in Mongolian medicine and Mongolian medicine de-termination method.

关 键 词：哈斯-哈图古日-15 没食子酸 HPLC 

分 类 号：R291.2[医药卫生—民族医学]