兰州百合鳞茎再生繁殖体系的建立  

Regeneration propagation system establishment of bulblet derived from scale of Lilium davidii var. Unicolor(Hoog) Cotton

作　　者：韦莉萍[1] 韦绍龙[2] 苏宾[2] 闭志强[2] 韩沅杉 张进忠[2] 

机构地区：[1]广西农业科学院农业科技信息研究所,南宁530007 [2]广西农业科学院生物技术研究所,南宁530007

出　　处：《南方农业学报》2014年第5期742-748,共7页Journal of Southern Agriculture

基　　金：广西农业科学院基本科研业务专项项目(桂农科2014YQ15);南宁市科学研究与技术开发计划项目(20133164)

摘　　要：[目的]建立兰州百合鳞茎快繁体系,并测定丛芽与鳞茎的淀粉含量,为系统研究兰州百合鳞茎离体再生过程中淀粉的代谢奠定基础.[方法]以兰州百合鳞片为外植体,探讨不同消毒剂组合、激素与蔗糖用量对鳞茎诱导培养过程的影响.[结果]随着75％乙醇（10～30 s）与0.1％升汞消毒时间（7～10min）的延长,外植体鳞片污染数不断下降,但诱导芽数表现为先上升后下降的趋势,以75％乙醇消毒30 s＋0.1％升汞消毒10 min组合的消毒效果最好,外植体污染率和芽诱导率分别为12.33％和91.00％.使用1000倍多菌灵处理10 min后,再使用75％乙醇30 s ＋0.1％升汞7～13 min组合进行消毒,可明显降低鳞片污染率3.00％～5.00％（绝对值）.在MS＋0.03 mg/L NAA＋30.0 g/L蔗糖＋5.0 g/L琼脂培养基中添加6-BA 0.5～1.0 mg/L,可显著提高平均芽诱导数;在MS＋5.0 g/L琼脂培养基中添加30.0～60.0 g/L蔗糖,对外植体芽诱导无明显影响;培养基MS＋1.0 mg/L 6-BA＋0.03 mg/L NAA＋30.0 g/L蔗糖＋5.0 g/L琼脂是外植体芽诱导的最适培养基.MS＋0.50 mg/L 6-BA＋0.03 mg/L NAA＋30.0 g/L蔗糖＋5.0 g/L琼脂为适宜增殖培养基,芽增殖系数达最高,为3.67.在MS培养基中添加60.0～90.0 g/L蔗糖可明显促进鳞茎的形成,以添加90.0 g/L蔗糖处理的鳞茎重量最高（99.30mg）.小鳞茎的淀粉质量分数比丛芽增加62.34％.[结论]以鳞片为外植体建立的兰州百合鳞茎再生繁殖体系具有可行性;在丛芽至小鳞茎形成阶段淀粉含量明显升高,小鳞茎的形成与淀粉含量升高密切相关.[Objective]The present study was conducted to establish regeneration propagation system of bulblet derived from scale of Lilium davidii var.Unicolor （Hoog） Cotton,and determine starch content in cluster buds and bulbs in order to lay the foundation of starch metabolism researches during in vitro regeneration of bulblets.[Method]By using scale as explants,the effects of different disinfector combinations on contamination and induction of explants,and different hormones and sugar dosages on bulblets induction process were investigated.[Result]With the sterilizing time elongation of 75％ alcohol （10～30 s） and 0.1％ HgC12 （7-10 min）,the contamination rate of explants decreased gradually,while the buds induction rate increased firstly and then decreased.The suitable sterilizing combination for explants was 75％ alcohol 30 s＋0.1％ HgC12 10 min,which could get 12.33％ of contamination rate and 91.00％ of induction rate.Furthermore,after treating by 1000× carbendazim for 10 minutes,the scale contamination rate decreased 3.00％-5.00％ （absolute value） significantly by using combination of 75％ alcohol 30 s＋0.1％ HgC127-13 min.By adding 6-BA 0.5-1.0 mg/L to culture medium MS ＋0.03 mg/L NAA ＋30.0 g/L Sugar＋ 5.0 g/L Agar,the buds induction rate of explants was increased significantly.Adding 30.0-60.0 g/L in medium Sugar MS＋5.0 g/L Agar did not show any obvious effects on buds induction.The suitable buds induction medium was MS ＋1.0 mg/L 6-BA ＋0.03 mg/L NAA＋30.0 g/L Sugar＋5.0 g/L Agar.The multiplication coefficient of buds was found the highest （3.67） in suitable medium MS＋0.50 mg/L 6-BA＋0.03 mg/L NAA＋30.0 g/L Sugar＋5.0 g/L Agar.In MS medium adding 60.0-90.0 g/L sugar,the formation of bulblets could be promoted.The highest weight was 99.30 mg when added 90.0 g/L sugar in MS medium.The starch content of bulblets increased by 62.34％ with compared to that of cluster buds.[Conclusion]It was feasible to establish regeneration propagation system of Lilium davidii bulble

关 键 词：兰州百合 鳞片 鳞茎 繁殖体系 淀粉 

分 类 号：S682.29[农业科学—观赏园艺]