异丙酚对大鼠全脑缺血再灌注后海马Akt—FOXO3a信号通路的影响  

Effect of propofol on hippocampus Akt -FOXO3a signaling pathway in global cerebral ischemia and reperfusion rats

作　　者：闫东[1] 柏平[1] 

机构地区：[1]重庆医科大学附属永川医院麻醉科,重庆402160

出　　处：《中国急救医学》2014年第7期661-664,共4页Chinese Journal of Critical Care Medicine

摘　　要：目的探讨异丙酚对大鼠全脑缺血再灌注后海马Akt—FOXO3a信号通路的影响。方法采用四血管闭塞法建立全脑缺血模型（IR组），全脑缺血再灌注＋异丙酚组（IR＋P组）脑缺血前10min腹腔注射异丙酚，假手术组（S组）仅接受假手术。术后48h三组大鼠进行神经功能学检测。术后4h处死取大脑，SP法进行免疫组化以及Western blots检测大鼠海马Akt、P—Akt、FOX03a、P—FOXO3a蛋白的表达。结果与S组比较，IR组和IR＋P组运动功能和感觉功能均明显降低（P〈0．01），IR＋P组高于IR组（P〈0．05或P〈0．01）。免疫组化及Western blots检测结果显示，三组海马Akt和FOX03a蛋白表达量在术后比较差异无统计学意义（P〉0．05）；IR组和IR＋P组海马P—Akt蛋白表达量明显高于s组（P〈0．05或P〈0．01）；IR＋P组低于IR组（P〈0．05或P〈0．01）。IR组和IR＋P组海马P—FOXO3a蛋白表达量明显低于S组（P〈0．01），IR＋P组高于IR组（P〈0．05或P〈0．01）。结论异丙酚可能通过对全脑缺血再灌注大鼠海马区Akt—FOXO3a信号通路活化的抑制作用，起到保护大鼠神经功能的作用。Objective To investigate the effects of propofol on hippocampus Akt - FOXO3a signaling pathway of global cerebral ischemia and reperfusion rats. Methods Rat global cerebral ischemic model was established by four - vessel occlusion （IR group ）, IR ＋ P group rats got intraperitoneal injection of propofol 10 min before ischemia, sham operation group （S group） received only sham surgery. Neurological functions of three groups were detected 48 h after surgery. Rats were sacrificed and rat brains were obtained 4 h after surgery to detect protein expression of Akt, p - Akt, FOXO3a, p - FOXO3a in rat hippocampus by immunohistochemistry （SP method） and Western blotting. Results Sports functionality and sensory function of IR group and IR ＋ P group were significantly lower than S group （ P 〈 0. 01 ）, and were higher in IR ＋ P group than those in IR group （P 〈 0. 05 or P 〈0. 01 ）. Immunohistochemistry and Western blots results showed that the expression of Akt and FOXO3a in the three groups showed no significant difference （P 〉 0.05） ; the expression of p - Akt in IR group and IR ＋ P group were significantly higher than that in S group （ P 〈 0.05 or P 〈 0. 01 ）, and it was lower in IR ＋ P group than that in IR group （P 〈0.05 or P 〈0.01 ）. The expression of p - FOXO3a of IR group and IR ＋ P was significantly lower than that in S group （P 〈0.01 ）, and was higher in IR ＋ P group than that in IR group （P 〈 0.05 or P 〈 0. 01 ）. Conclusion Propofol possibly affects on hippocampus Akt - FOXO3a signaling pathway of global cerebral ischemia and reperfusion in rats and it plays a protective role in neurological function of rats.

关 键 词：全脑缺血再灌注 异丙酚 Akt—FOXO3a信号通路 神经功能 

分 类 号：R743.31[医药卫生—神经病学与精神病学]