聚腺苷二磷酸核糖聚合酶假基因多态性分布及其与肺癌易感性的关系  

Distribution of poly(ADP-ribose) polymerase pseudogene polymorphism and association with susceptibility to lung cancer in Chinese people

作　　者：唐录英[1] 任泽舫 庄志雄[2] 刘晓合 苏祖兰 黄钰[2] 

机构地区：[1]中山医科大学肺癌研究中心 [2]中国预防医学科学院深圳研究中心

出　　处：《中华医学遗传学杂志》2002年第2期124-126,共3页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金 (30 0 70 674)~~

摘　　要：目的　研究聚腺苷二磷酸核糖聚合酶假基因多态性在汉族人群中的分布 ,探讨该等位基因在衡量肺癌易感性方面的意义。方法　肺癌患者 6 3例 ,与病例在年龄、性别等方面匹配的健康对照 82名 ,抽提外周血白细胞基因组 DNA,特定引物 PCR扩增 ,在含溴乙锭的琼脂糖凝胶中电泳 ,紫外光下观测和成像。结果　病例组和对照组的基因型分布差异无显著性 ,B等位片段频率分别为 0 .0 95和 0 .116 ;无论是否含有 B基因 ,吸烟都是肺癌的危险因素 (P<0 .0 5 ) ,基因型为 AA时 ,吸烟的比值比 (odds ratio,OR)是2 .2 8,基因型为 AB或 BB时 ,其 OR则达 4.83;在不吸烟者中 ,AB或 BB基因型者患肺癌的危险性并未增高 (P=0 .2 0 2 )。结论　中国汉族人群 B等位片段频率较其他民族低 ,是肺癌的可能易感标记 ,但只在吸烟者才表现出来 。Objective To study the distribution of poly(ADP-ribose) polymerase(PARP) pseudogene polymorphism and the association with susceptibility to lung cancer in Chinese people. Methods The subjects of this study included 63 patients with lung cancer and 82 healthy controls matched in gender and age. Genome DNA was extracted from white blood cells.Products from PCR with a pair of specific primer were electrophoresized in agarose including EB. Under ultraviolet, observation and imaging were performed.Results There was no significant difference in genotype between the cases and controls. The frequencies of B allele in cases and controls were 0.095 and 0.116 respectively. Whether there was B allele or not, smoking was a risk factor of lung cancer( P<0.05) . As the genotype was AA and AB or BB, smoking OR was 2.28 and 4.83 respectively. Among non-smokers, the risk at lung cancer did not increase in AB or BB genotypes( P=0.202) . Conclusion Frequency of B allele is relatively lower in Chinese people than in other races. In smokers, B allele may be a susceptible marker of lung cancer, and there is synergistic function between B allele and smoking.

关 键 词：聚腺苷二磷酸核糖聚合酶 基因多态性 肺癌 易感性 分布 

分 类 号：R734.2[医药卫生—肿瘤]