隔离群体中3个Y-STR基因座的遗传多态性分析  

Analysis of three Y-STR loci polymorphism in isolated populations

作　　者：蔡善荣[1] 李鲁 柯越海[3] 刘希永 曹江[2] 卢大儒[3] 郑树[2] 

机构地区：[1]浙江大学医学院附属第二医院,杭州310009 [2]浙江大学医学院肿瘤研究所,杭州310009 [3]复旦大学遗传所

出　　处：《中华医学遗传学杂志》2002年第2期141-144,共4页Chinese Journal of Medical Genetics

基　　金：国家人类基因组南方研究中心基金 (CNCS- 99M- 0 2 )~~

摘　　要：目的　了解 3个 Y染色体上短串联重复序列 (short tandem repeat on Y chromosome,Y-STR)基因座在隔离群体中的多态性 ,探讨用 Y- STR分析群体亲缘关系的可能性。方法　在 377测序仪上应用基因扫描技术对浙江省两个海岛隔离群体 (80人和 6 0人 ) ,和 1个开放群体 (36人 )的 DYS388、DYS390、DYS395进行检测。结果　 DYS388、DYS390、DYS395在渔山岛、桃花岛、开放群体中的等位基因数分别为 8、9、7;5、6、7;6、6、5。 3个群体中每个 Y- STR基因的基因多样性在 0 .70～ 0 .80之间。隔离群体和开放群体在等位基因的频率分布、相同基因型数的分布差异无显著性。遗传距离最大的为桃花岛与开放群体 ,其次是渔山岛与桃花岛群体 ,最小的是渔山岛和开放群体。结论　 DYS388的常见等位基因为 12 9,DYS390、DYS395分别为 2 15和 119。 DYS388、DYS390、DYS395的基因频率、基因多样性、群体的相同基因型分布和遗传距离均不能说明隔离群体之间、以及与开放群体的亲缘关系程度 ,提示用 Y- STR分析群体亲缘关系须在增加样本量的基础上进行继续深入的研究。Objective To figure out the polymorphism of three Y-STR loci in isolated populations and explore the consanguinity of the populations with the use of Y-STR. Methods Male samples were selected from two isolated populations(80 and 60 males) in Zhejiang province and one open population (36 males), genescan was performed with males' DNA by genescan technology with ABI PRISM 377 sequencer at Y chromosome loci DYS388, DYS390 and DYS395. Results DYS388,DYS390, DYS395 allele counts in Yushan island population, Taohua island population and open population were 8,9,7;5,6,7;and 6,6,5 respectively. Gene diversity was between 0.70-0.80 in the three populations. There was no difference in distribution of allele frequency and shared genotypes between the isolated populations and the open population by statistical test. Genetic distance is long between Taohua island population and open population, short between Yushan island population and open population, and moderate between Yushan island population and Taohua island population. Conclusion The main allele is 129 at DYS388; 215 at DYS390; and 119 at DYS395. The distribution of allele frequency and gene diversity at DYS388, DYS390, DYS395 loci, and the shared genotypes between populations as well as the genetic distance are unable to explain the blood relationship between the isolated and open populations, suggesting the additional studies in large sample size will be necessary to use Y-STR for exploring the blood relationship between populations.

关 键 词：Y染色体 短串联重复序列 遗传多态性 隔离群体 遗传距离 基因扫描 

分 类 号：R394[医药卫生—医学遗传学]