聚集素在前列腺癌中的表达及意义  

Expression and significance of clusterin in prostate neoplasm

作　　者：周利群[1] 杨学贞[1] 梁丽莉[1] 张强[1] 李钟[2] 张志文[1] 那彦群[1] 郭应禄[1] 

机构地区：[1]北京大学第一医院泌尿外科北京大学泌尿外科研究所,100034 [2]美国芝加哥西北大学泌尿分子生物学系

出　　处：《中华泌尿外科杂志》2002年第3期181-183,共3页Chinese Journal of Urology

基　　金：国家自然科学基金 (3 980 0 173 );国家教委留学归国人员科研启动基金 (98 2 0 0 0 )

摘　　要：目的　探讨抗细胞凋亡因子聚集素 (clusterin)在前列腺癌中的表达及意义。　方法　RT PCR方法检测聚集素在前列腺癌组织 (3例 )、癌细胞系 (1株 )及正常前列腺组织 (3例 )中的表达水平。　结果　 3例前列腺癌组织及 1例前列腺癌细胞株中聚集素与内参基因 β actin相比较的相对表达量明显高于 3例正常前列腺组织。　结论　聚集素在前列腺癌中高表达 ,提示聚集素可能通过抗凋亡机制在前列腺癌的生物特性中发挥作用。Objective To study the expression and significance of antiapoptosis facter-clusterin in prostate neoplasm. Methods Reverse transcriptive polymerase chain reaction(RT PCR) was used to examine the expression level of clusterin in prostate cancer tissues(3 cases),prostate cancer cell line PC3M and normal prostate tissues(3 cases). Results Compared with the internal marker gene β actin , the expression level of clusterin in prostate cancer is much higher than that of normal prostate. Conclusions The high expression level of clusterin in prostate cancer indicates that clusterin may play an important role in the biological characteristics of prostate cancer through the antiapoptosis pathway.

关 键 词：前列腺癌 聚集素 逆转录聚合酶链反应 细胞凋亡 实验研究 

分 类 号：R737.25[医药卫生—肿瘤]