乙型肝炎、性病和HIV-1感染人群中CCR5,CCR2和SDF1等位基因多态性的研究  

Genotyping and distribution of mutant CCR5△32, CCR2-641 and SDF1-3 ' A alleles in HIV-1 carriers and patients with sexually-transmitted diseases and chronic HBV infection

作　　者：刘惠刚[1] 王福生[2] 洪卫国[2] 金磊[2] 周越塑 侯静[2] 张冰[2] 刘明旭[2] 

机构地区：[1]北京职工医学院基础医学系,北京市100036 [2]中国人民解放军302医院生物工程研究室,北京市丰台路26号100039

出　　处：《世界华人消化杂志》2002年第1期19-23,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助;No.397706830

摘　　要：目的:研究乙型肝炎、HIV-1感染者和性病患者中CCR5,CCR2和SDF1等位基因的分布及其特点。 方法:收集乙型肝炎患者(268例)、艾滋病感染者(130例)和性病(259例)的血液标本共657份,应用QIAGEN全血细胞基因组提取试剂盒,分离纯化人血细胞基因组DNA样品,应用PCR或PCR-RFLP分析CCR5,CCR2-64I和SDF1-3’A等位基因突变频率和分布特点。 结果:在乙型肝炎、性病和HIV-1感染者人群中,CCR5△32的突变频率分别为0.19％、0％和0％,在259例个体性病患者中只有一例男性发生了杂合子突变,而在乙型肝炎和HIV-1感染者人群中没有检测出CCR5△32突变基因,可见,CCR5△32的突变率较低,反之,CCR2-64I的突变频率较高,与前述三种患者对应的突变频率分别为19.3％、19.6％和20.7％,进而分析发现SDF1-3’A等位基因在乙肝、性病和HIV-1感染人群中的突变率分别为27.8％,27.4％和26.9％,应用x^2检测与统计学分析显示在乙肝,性病和HIV-1感染人群中CCR2-64I和SDF1-3’A等位基因突变分布之间无连锁关系。 结论:突变频率很低的CCR5△32基因型在上述3种类型传染病的发病过程中可能不起主要作用;而突变频率较高的CCR2-64I和SDF1-3’A基因型的功能及其在上述3种传染病进程中的意义有待进一步研究。AIM:To evaluate the polymorphisms of mutant CCR5△32, CCR2-641 and SDF1-3' A alleles in HIV-1 carriers and patients with sexually-transmitted disease (STD) and chronic HBV infection from Han ethnic population in China.METHODS: The genomic DMAs from all 657 patients were purified from whole peripheral blood samples of HIV-1 carriers (n = 130), patients with (n = 259) and the patients with chronic HBV infection (n = 268) using QIAamp DNA Blood Mini Kit. The genotyping and distribution of CCR5△32, CCR2-641 and SDF1-3' A alleles were further identified by means of PCR or PCR-RFLP assays and statistical analyses.RESULTS:The mutation frequency of CCR5△32 alleles was 0%, 0. 19% and 0% for HIV-1 carriers, STD and HBV-infected patients, respectively, which is similar to the features with a very low frequency of CCR5△32 mutant in healthy individuals from Chinese Han population as previously reported. Comparatively, there was a higher frequency of CCR2-641 genes of 19.62%, 19.31% and 20. 7%, and of SDF1-3'A alleles of 27.8%, 27.4% and 26. 9%, respectively, in the corresponding patients of aforementioned three groups. Statistical analyses showed that there are independent distributions of the three CCR5△32, CCR2-641 and SDF1-3' A alleles in the HIV-1 carriers, HBV-infected patients and the STD patients.CONCLUSIONS: The evidence of very low frequency of mutant CCR5△32 allele suggested that this polymorphism is unlikely to play a major role as a host genetic factor, but the functions and its implications of higher frequency of CCR2-641 and SDF1-3' A alleles remain to be further clarified in HIV-1 carriers as well as in STD cases and HBV-infected patients.

关 键 词：乙型肝炎 性病 HIV-1感染人群 CCR5 CCR2 SDF1 基因多态性 

分 类 号：R512.62[医药卫生—内科学] R759[医药卫生—临床医学]