六亚甲基双乙酰胺与紫杉醇合用对人骨肉瘤OS-9901细胞的抑制作用  

Inhibitory effects of hexamethylene bisacetamide combined with taxol on human osteogenic sarcoma cell line OS-9901

作　　者：俞玮[1] 张殿忠[1] 范清宇[1] 周勇[1] 

机构地区：[1]第四军医大学唐都医院解放军骨科中心骨肿瘤研究所,陕西西安710038

出　　处：《医学研究生学报》2002年第2期112-114,共3页Journal of Medical Postgraduates

摘　　要：目的 :研究六亚甲基双乙酰胺 (HMBA)与紫杉醇合用对人骨肉瘤OS 990 1细胞的抑制作用。　方法 :用MTT法检测HMBA、紫杉醇单用或二者合用对体外培养的人骨肉瘤OS 990 1细胞增殖变化的影响。　结果 :在单用紫杉醇处理组中 ,不同药物浓度均未使增殖细胞抑制达到生长半数。在先用 1.0 μg/ml紫杉醇处理 6h后再加用 1mmol/LHMBA组中 ,抑制率由 18%增至 5 9%。 0 .1μg/ml紫杉醇先处理 2 4h组中 ,抑制率由 2 3 %增至 67%。HMBA明显增强了紫杉醇的抗癌作用。　结论 :HMBA与紫杉醇联合应用对骨肉瘤保肢术后的治疗可能有一定的价值。Objectives:To study the inhibitory effect of hexamethylene bisacetamide (HMBA)combined with taxol on human osteogenic sarcoma cell line OS 9901. Methods:MTT assay was used to determine the inhibitory effects of HMBA, taxol and HMBA combined with taxol on human osteogenic sarcoma cell line OS 9901 in vitro . Results: In the taxol groups, the inhibited growth rates of osteogenic sarcoma cells in different concentrations were all below 50%. After combining with 1mmol/L HMBA, the inhibited growth rate was increased from 18% to 59% and 23% to 67% when cells were continuously exposed to 1.0 μg/ml of taxol for 6 h and 0.1 μg/ml for 24 h. HMBA can enhance taxol cytotoxicity. Conclusions: HMBA combined with taxol may provide a new approach to osteogenic sarcoma therapy.

关 键 词：骨肉瘤 分化诱导 六亚甲基双乙酰胺 紫杉醇 治疗 抑制作用 

分 类 号：R738.105[医药卫生—肿瘤]