表达序列标签数据库搜索鉴定小鼠UBAP1基因及其数字化表达分析  

Identification and Digitalized Expression Analysis of Murine UBAP1 Gene by Means of EST Database Searching

作　　者：钱骏[1] 董利[1] 张必成[1] 王洁如[1] 周鸣[1] 李忠花[1] 李伟芳[1] 李小玲[1] 李桂源[1] 

机构地区：[1]中南大学湘雅医学院肿瘤研究所

出　　处：《生物化学与生物物理进展》2002年第2期323-327,共5页Progress In Biochemistry and Biophysics

基　　金：国家高技术"86 3"计划资助项目 ( 10 2 10 0 1 0 5 ;Z19 0 1 0 1 0 3);国家重点基础研究发展规划项目 ( 973) (G19980 5 10 0 8);美国中华医学基金资助 ( 96 6 5 5 )~~

摘　　要：UBAP1(ubiquitinassociatedprotein 1)基因是最近克隆的一个定位于人类染色体 9p2 1 2 2鼻咽癌杂合性丢失高频区的泛肽相关蛋白家族新成员 .为了深入研究UBAP1基因的功能 ,利用计算机对表达序列标签(expressedsequencetag ,EST)、UniGene等数据库进行综合搜索分析 ,结合cDNA克隆测序的方法 ,成功地获得了UBAP1基因在小鼠中的同源基因 .小鼠UBAP1基因cDNA全长为 2 6 76bp ,编码一个由 44 1个氨基酸组成的蛋白质 ,在其蛋白质C端只有一个泛肽相关功能域 (UBAdomain) .与人UBAP1基因相比 ,两者编码的氨基酸序列有 89%相同 .基于EST的数字化表达分析显示UBAP1基因在小鼠正常组织中广泛高表达 .Ubiquitin associated protein 1 (UBAP1) is a novel member of UBA domain protein family, located at human chromosome 9p21-22 where loss of heterozygosity frequently occurs in nasopharyngeal carcinoma. Through integrated analysis of public database such as EST, Unigene in silico and direct sequencing of cDNA clone, full-length cDNA sequence of murine homologoue of human UBA-P1 gene was identified, which is 2 676 bp long and encodes a putative protein of 441 amino acids with a UBA domain. There is an average of 89% identical residues between mouse and human UBAP1 protein. Digitalized expression analysis based on EST data showed that mouse UBAP1 gene expressed ubiquitiously and strongly in most of mouse normal tissues.

关 键 词：表达序列标签 数据库搜索 小鼠 UBAP1基因 数字化表达分析 鼻咽癌 组织表达 基因克隆 

分 类 号：R394.8[医药卫生—医学遗传学] R739.63[医药卫生—基础医学]