海葵毒素多肽的分离和初步表征  

Isolation and Characterization of Toxin Polypeptides fromSea Anemone Actinia cari

作　　者：禚如朋[1] 付宏征[1] 李军[1] 张礼和[1] 林文翰[1] 仲崇波[2] 王晓良[2] 

机构地区：[1]北京大学天然药物及仿生药物国家重点实验室,北京100083 [2]中国医学科学院药物研究所,北京100050

出　　处：《高等学校化学学报》2002年第4期605-609,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:30171106)资助.

摘　　要：经冻融法提取,丙酮沉淀,ＳＰ Ｓｅｐｈａｄｅｘ Ｃ－２５强离子交换和ＨＰＬＣ ＯＤＳ反相色谱层析,从海葵(Ａｃ－ｔｉｎｉａｃａｒｉ)中提纯到２个新的毒素多肽,命名为ＲＳＡＰⅢ和ＲＳＡＰ Ⅳ．经ＳＤＳ－聚丙烯酰胺凝胶电泳(ＳＤＳ－ＰＡＧＥ)和基质辅助激光解吸电离飞行时间质谱(ＭＡＩ－ＤＩＴＯＰ－ＭＳ)鉴定,ＲＳＡＰⅢ和ＲＳＡＰ Ⅳ均为单一成分,分子量分别为５ ３２７和５ ３１０,初步钠通道药理学实验表明,ＲＳＡＰⅢ对豚鼠心室肌细胞钠通道表现出活性,使钠离子内流减少,起阻滞作用．Two toxin polypeptides desgnated RSAPⅢ and RSAP Ⅳ were isolated from sea anemone Ac-tinia cari. Toxins isolation was accomplished by freezing-thawing extraction, fractional acetone precipita-tion, cation exchange chromatography on SP Sephadex C-25 and reverse phase HPLC on ODS C18. Theywere found to be homogenous by SDS-PAGE, and their moleculax weight at m/z 5 327 and m/z 5 310 re-spectively were determined by MALDI-TOF-MS. RSAP Ⅲ possessed a bioactivity on sodinm channel inGuinea pig cardiac cell to inhibit inward Na+ currents, which is contrary to that of all sea anemone toxinsisolated so far.

关 键 词：海葵 海葵毒素 分离 纯化 表征 多肽 动物药 有效成分 

分 类 号：R284.2[医药卫生—中药学]