Δ6去饱和酶催化合成γ-亚麻酸  

△6 Desaturase Catalyzing Linoleic Acid to γ-Linolenic Acid

作　　者：咸漠[1] 刘延[1] 李文兴[1] 毕颖丽[1] 甄开吉[1] 康亦兼[2] 闫吉昌[3] 

机构地区：[1]吉林大学化学学院,长春130023 [2]青岛化工学院化工系,青岛266042 [3]东北师范大学化学学院,长春130021

出　　处：《高等学校化学学报》2002年第4期624-627,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:29873018)资助.

摘　　要：报道了含Δ6去饱和酶的深黄被孢霉菌丝提取物催化亚油酸合成γ-亚麻酸. 研究了辅酶、温度、时间等对γ-亚麻酸合成的影响. 结果表明, 反应温度降低, γ-亚麻酸的产率提高. 在10 ℃以下, γ-亚麻酸的产率达到最大值; 苹果酸盐对该反应有明显促进作用. γ-亚麻酸的产率随体系pH增加而增加, 且在pH=7～8时产率较高; 该反应需在分子氧存在下进行; NADPH, ATP和CoA是该反应的必需辅酶. 在优化的反应条件下, γ-亚麻酸的产量达到0.21 mg/mL.The biosynthesis of γ-linolenic acid from linoleic acid with △6 desaturase, Morierella isabellina,is reported. Various factors affecting this process were studied. The results show that the yield of the γ-linolenic acid increases with the decrease of the reaction temperature and reachs a maximum when incubat-ed below 10℃. Adding malate to the reaction mixture caused a marked increase of γ-linolenic acid yield;an increased linolenic acid yield could also be obtained with the increase of pH value of the reaction mixtureand reached the maximum at pH 7-8. Oxygen was an essential substrate, and NADPH, CoA and ATPwere inpartent cofactors for the reaction. The yield of γ-linolenic acid reached 0.21 mg/mL under the opti-mal reaction conditions.

关 键 词：△6去饱和酶 Γ-亚麻酸 生物合成 深黄被孢霉 催化合成 

分 类 号：O623.612[理学—有机化学] Q939.9[理学—化学]