血清VEGF检测及卵巢肿瘤组织VEGFp53表达与卵巢上皮肿瘤相关性分析  

A correlation analysis between the serum level of vascular endothelial growth factor (VEGF),expression of VEGF,p53 in ovarian tumor tissues and the epithelial ovarian carcinoma

作　　者：孙立新[1] 焦士兰[1] 王全红[2] 

机构地区：[1]山西省肿瘤医院妇科,030013 [2]山西省肿瘤医院病理科,030013

出　　处：《中国药物与临床》2002年第2期80-83,共4页Chinese Remedies & Clinics

摘　　要：目的　通过对卵巢上皮肿瘤患者血清血管内皮生长因子 (VEGF)水平的检测及对卵巢上皮肿瘤组织中VEGF、p5 3表达的观察 ,以探讨VEGF、p5 3与卵巢上皮肿瘤临床病理诸因素的关系。方法　用酶联免疫吸附试验 (ELISA)法测定 71例卵巢上皮肿瘤患者术前血清及 10例腹水中VEGF水平 ,其中对 7例卵巢上皮癌患者血清进行了连续检测 ;用免疫组织化学检测 73例卵巢上皮肿瘤组织中VEGF、p5 3的表达。结果　①在卵巢上皮肿瘤患者中 ,血清VEGF水平在良性组、交界性组及恶性组差异有显著性 ;在恶性组中 ,血清VEGF水平与临床期别、病理分级有明显相关性 ,与病理类型无明显相关性 ;卵巢癌患者经肿瘤减灭术后 ,血清VEGF均有不同程度下降 ,而当病情复发后 ,血清VEGF再度上升。②VEGF在卵巢上皮肿瘤组织中的表达 ,恶性组阳性表达率 (86 4 % )明显高于交界性组 (6 6 7% ,P <0 0 0 5 )及良性组 (37 5 % ,P <0 0 0 5 ) ;在恶性组中 ,VEGF强阳性表达率 ,分化差的G3组 (42 1% )高于分化好的G1 、G2 组 (30 4 % ) ,但差异无显著性 (P >0 0 5 ) ;临床分期差异也无显著性 (P >0 0 5 )。③p5 3在卵巢上皮肿瘤组织中的表达 ,恶性组阳性表达率 (76 2 % )明显高于交界性组 (2 8 6 % ,P <0 0 1)及良性组 (9 1% ,P <0 0 1)。Objective To evaluate the level of serum VEGF,and VEGF,p53 expression in patients with ovarian epithelial carcinoma.Methods Serum VEGF was detected in 71 patients including 42 patients with epithelial ovarian carcinoma (EOC),7 patients with borderline epithelial ovarian tumor (BOT),and 22 patients with benign epithelial ovarian tumor (BET);20 healthy women as control group (CON);7 patients with EOC were continuously determined before and after operation.Enzyme linked immunosorbent assay (ELISA) was utilized to measure the surum VEGF.VEGF and p53 expression in epithelial ovarian tumor was analysed immunohistochemically in 73 patients.Results ① Serum VEGF levels before operation in patients with EOC were significantly higher than those with BOT,BET and CON;in EOC serum VEGF levels in patients at advanced stage,low differentiation was apparently higher than those at early stage ( P <0 05).Serum VEGF levels decreased after the successful removal of tumor in ovarian cancer patients,and in three patients,the serum VEGF levels were re elevated during relapse.② Positive immunostaining for VEGF was observed in 86 3% of EOC,which was significantly higher than that of BOT 66 7% ( P <0 005) and BET 37 5% ( P <0 005).③ Postive immunostaining for p53 was observed in 76 2% of EOC,which was significantly higher than that of BOT 28 6% ( P <0 01) and BET 9 1% ( P <0 01),in EOC p53 expression in patients at advanced stage was higher than that at early stage ( P <0 01).VEGF expression and p53 expression in patients with epithelial ovarian tumor showed positive correlation,the correlation was 0 438 32 ( P <0 01).Conclusions ① The elevation of seum VEGF levels and increase of VEGF,p53 expression in EOC were all associated with the malignent behavior.② p53 expression in epithelial ovarian tumor was associated with VEGF expression.③ Serum VEGF could be used as a marker for montoring epithelial ovarian carcinoma in clinic.

关 键 词：血管内皮生长因子 酶联免疫吸附测定 免疫组织化学 卵巢上皮肿瘤 P53基因 血清 

分 类 号：R737.31[医药卫生—肿瘤]