赤芝菌丝体活性多糖的分离、纯化及结构研究  

Isolation,Purification and Structural Characterization of Active Polysaccharides from the Mycelium of Ganoderma lucidum

作　　者：赵长家[1] 何云庆[1] 

机构地区：[1]北京大学药学院,北京100083

出　　处：《中药材》2002年第4期252-254,共3页Journal of Chinese Medicinal Materials

摘　　要：赤芝(Ganoderma lucidum)固体发酵菌丝体经水堤、乙醇沉淀得总菌丝体多糖,药理实验表明,总多糖具有明显的增强免疫和抗肿瘤活性。总多糖经DEAE-Sephadex柱层析分离、脱色、脱盐得多糖 GLMB_0。总多糖经硼酸型DEAE-Cellulose柱层析分离、脱色、脱盐得多糖GLMB_1。Sephadex柱层析法和旋光法测定了两多糖的纯度。GLMB_0为肽杂多糖,GLMB_1为杂多糖。笔者通过IR、完全酸水解、GC、Smith降解、酶解、β-消除反应、CNMR对GLMB_1、GLMB_0进行了结构研究。The active polysaccharides from the mycelium of Ganoderma lucidum were fractioned by DEAE-Sephadex and DEAE-Cellu-lose.Through repeated isolation and purification,two polysaccharides, GLMB0 and CLMB1, were finally obtained.Their structures were characterized by chemical and spectral methods.

关 键 词：赤芝 菌丝体 活性多糖 分离 纯化 结构 研究 

分 类 号：R284.2[医药卫生—中药学]