癌宁诱导人胃腺癌细胞SGC-7901凋亡的形态学变化及未端标记  

Apoptotic Morphological Changes and TUNEL of Human Gastric Cancer Cell SGC-7901 Induced by Aining

作　　者：苏勉诚 戴梅 吕晓英[3] 刘继林[4] 李红坤[4] 

机构地区：[1]广东省珠海市国家中药现代化工程技术研究中心,珠海519020 [2]重庆市沙坪区中医院,400030 [3]四川大学公共卫生学院,成都610041 [4]成都中医药大学,610075

出　　处：《中药材》2002年第4期270-272,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:探索癌宁对人胃癌细胞的诱导凋亡作用。方法:采用体外细胞培养技术,通过形态学、分子生物学方法,对中药复方癌宁和顺铂诱导胃癌细胞凋亡进行了深入研究。结果:经癌宁及顺铂处理后的细胞出现凋亡形态学改变;癌宁在1 g/L浓度对细胞的凋亡诱导率是46.36％,与25 mg/L顺铂对细胞的凋亡诱导率49.12％间无显著差异(P>0.05),两者均与空白对照组有显著差异(P<0.01)。结论:癌宁及顺铂对人胃癌细胞具有诱导凋亡作用,中药复方癌宁可望成为一种有前途的新的癌细胞凋亡诱导剂。Objective: To investigate the inducing apoptosis effect of Aining on the human gastric cancer cell SGC-7901. Methods: In vitro cell culture was adopted to explore the inducing apoptosis efiect of Aining and cisplation (DDP) through the morphological method and the molecular method.Results: Apoptotic morphological changes were seen after the cells cultured with Aining or DDP;apoptosis rate of 1 g/L Aining group was 46.36%, which had no significant differences with the apoptosis rate 49.12% of the 25 mg/L DDP group; both of the Aining and the DDP groups were significantly different from that of the blank group (P < 0.01). Conclusions: Both DDP and Aining could induce the apoptosis of human gastric cancer cell and the traditional medicine compound prescription Aining is likely to become a new agent which is used to induce apoptosis of cancer cells.

关 键 词：癌宁 胃腺癌细胞 SGC-7901 形态学变化 末端标记 中药复方 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]