溃疡性结肠炎的白细胞介素(IL)-1β、IL-1受体拮抗剂、IL-4基因多态性  

Interleukin (IL)-1β,IL-1 receptor antagonist and IL-4 gene polymorphisms in ulcerative colitis in the Chinese

作　　者：彭仲生[1] 胡品津[1] 崔毅[1] 李初俊[1] 

机构地区：[1]中山医科大学附属第一医院消化内科,广州510080

出　　处：《中华内科杂志》2002年第4期248-251,共4页Chinese Journal of Internal Medicine

基　　金：广东省医学科学技术研究基金资助 (A2 0 0 0 15 5 )

摘　　要：目的　研究溃疡性结肠炎 (UC)病人的白细胞介素 (IL) 1β、IL 1受体拮抗剂 (IL 1RA)、IL 4基因多态性 ,并分析其与抗中性粒细胞胞质抗体 (ANCA)及临床分型的关系。方法　用PCR 限制性片段长度多态性方法和序列特异性引物 PCR方法分别对 81例UC病人和 114例健康者进行IL 1β和IL 1RA、IL 4基因多态性分析。结果　UC组IL 4的RP2基因频率明显高于对照组 (2 9 0 %对 11 8% ,P =0 0 0 0 0 2 ) ,而对照组RP1基因频率明显高于UC组 (88 2 %对 71 0 % ,P =0 0 0 0 0 2 ) ,UC组RP1 2、RP2 2基因型的优势比值分别为 2 71(95 %CI,1 39～ 5 31) ,9 0 4 (95 %CI,1 0 5～ 2 0 3 2 9) ;2组IL 1β、IL 1RA各基因频率比较差异均无显著性 (P >0 0 5 ) ;ANCA(+)组和ANCA(- )组UC病人IL 4的RP1、RP2基因频率显著不同 ,差异有显著性 (P值均 <0 0 5 )。结论　中国汉族UC病人与IL 4内含子 3的基因多态性相关联 ,UC病人IL 4RP1基因频率明显降低 ,而RP2基因频率明显增加 ,与正常人的差异发生在ANCA(+)UC病人 ;中国汉族UC病人与IL 1β、IL 1RA基因多态性无关联。Objective To study whether the Chinese ulcerative colitis (UC) patients are associated with interleukin (IL) 1β, IL 1RA and IL 4 gene polymorphisms and to analyze the relationship among gene polymorphisms, antineutrophil cytoplasmic antibodies (ANCA) and clinical classification of UC. Methods eighty one UC patients and 114 healthy subjects (HS) were enrolled. IL 1β, IL 1RA, and IL 4 gene polymorphisms were analyzed with polymerase chain reaction restriction fragment length polymorphism and polymerase chain reaction with sequence specific primers respectively. Results The gene frequency of allele RP2 of IL 4 in UC patients was significantly higher than that in HS (29.0% vs 11.8%, P = 0.000 02), but the gene frequency of allele RP1 in HS was significantly higher than that in UC patients (88.2% vs 71.0%, P = 0.000 02). OR of the genotype RP1.2 and RP2.2 were 2.71 (95%CI 1.39～5.31) and 9.04 (95%CI 1.05～203 29) respectively. There was not difference in the gene frequencies of IL 1β, IL 1RA between UC patients and HS ( P >0.05). When UC patients were divided into ANCA positive and negative groups, it was found that there was significant difference in the gene frequencies of allele RP1, RP2 of IL 4 between two groups (63.7% vs 83.3%, 36.3% vs 16.7%, P <0.05). Conclusions There is correlation between the Chinese UC patients and the gene polymorphisms of intron 3 of IL 4. The gene frequency of allele RP1 in UC patients are lower, but the gene frequency of allele RP2 is significantly higher. The differences on gene frequencies of IL 4 between the UC patients and HS are mainly found in the ANCA positive UC patients. The Chinese UC patients are not associated with IL 1β and IL 1RA gene polymorphisms.

关 键 词：溃疡性结肠炎 白细胞介素类 基因多态性 白细胞介素-1受体拮抗剂 

分 类 号：R574.62[医药卫生—消化系统]