p63、增殖细胞核抗原和表皮生长因子受体在皮肤鳞状细胞癌中的表达  

Expression of p63?PCNA and EGFr in Skin Squamous Cell Carcinoma

作　　者：王建力 陈莉[1] 王桂兰[1] 曹晓蕾[1] 

机构地区：[1]南通医学院病理教研室

出　　处：《中华皮肤科杂志》2002年第2期143-145,共3页Chinese Journal of Dermatology

基　　金：江苏省科委资助(Bs99358)

摘　　要：目的探讨皮肤鳞状细胞癌(SCC)中p63、增殖细胞核抗原(PCNA)和表皮生长因子受体(EGFr)的表达及其与癌增殖和分化的关系。方法采用免疫组化(LSAB)方法分析68例SCC中p63、PCNA和EGFr的表达。结果在正常和癌变上皮中p63、PCNA主要表达于具有增生潜能的基底细胞,而在终末分化的角质层细胞中缺如。高分化癌中p63呈环状分布于癌巢周围,低分化癌中p63阳性细胞增多,分布紊乱。癌中PCNA、EGFr表达于整个癌巢中。p63、PCNA、EGFr表达在SCC表皮内肿瘤(SIN)Ⅲ级中均明显高于正常或癌旁上皮,并在癌的不同分化中差异有显著性。在SCC中p63、PCNA、EGFr表达之间均有显著相关性。结论在SCC中由免疫组化显示p63、PCNA和EGFr过表达的癌细胞常是获得了更具增殖、侵袭和间变能力的细胞。Objective To investigate expression of p63?PCNA and epidermal growth factor receptor(EGFr)in skin squamous cell carcinoma(SCC) . Method Tissue specimens from 68 cases of SCC were studied using immunohistochemistry staining method with monoclonal antibodies to p63?PCNA and EGFr. Results The expression of p63 protein and PCNA was principally restricted to basal cells with high proliferative potentiality and was absent in cells of stratum corneum that were undergoing terminal differentiation in normal or carcinoma epidermis. In highly differentiated tumors, the staining of p63 formed a ring surrounding carcinoma nests. In low differentiated tumors, p63 positive cells were increased and disorderly arranged. Diffuse expression of PCNA and EGFr was found through out all carcinoma nests. Expression of p63?PCNA and EGFr was significantly stronger in SCC and intraepithelial neoplasm(SIN)level Ⅲthan those in normal or proliferative epidermis near the carcinoma. A significant correlation was found between expression of p63,PCNA,EGFr and degree of differentiation of SCC,and between p63 and EGFr,p63 and PCNA,PCNA and EGFr, respectively. Conclusion Overexpression of p63?PCNA?EGFr may be contributed to acquisition of enhanced capability of proliferation, aggression and anaplasia in SCC.

关 键 词：P63 增殖细胞核抗原 表皮生长因子受体 皮肤鳞状细胞癌 基因表达 

分 类 号：R739.5[医药卫生—肿瘤]