食管鳞癌中HPV-16/18感染与c-erbB-2、p53和Rb表达及其意义  

Infection of HPV-16/18,Expression and Significance of c-erbB-2,p53 and Rb in Esophageal Carcinoma

作　　者：牛永东[1] 李恩民[1] 吴贤英[2] 郑瑞明[2] 沈忠英[3] 许丽艳[3] 

机构地区：[1]汕头大学医学院生物化学与分子生物学教研室,广东汕头515031 [2]汕头大学医学院病理教研室,广东汕头515031 [3]汕头大学医学院肿瘤病理研究室,广东汕头515031

出　　处：《肿瘤防治杂志》2002年第2期138-141,共4页China Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金项目 ( 3990 0 0 6 9和 30 170 42 8) ;广东省自然科学基金资助项目 ( 990 799和 0 10 431);广东省高校自然科学科研项目 ( 2 0 0 0 33)

摘　　要：目的 :探讨食管鳞癌组织中人乳头瘤病毒HPV 16 / 18感染与c erbB 2、p5 3和Rb表达及其意义。方法 :采用聚合酶链反应 (PCR)法检测 90例活检存档的食管鳞癌蜡块中HPV 16 / 18的感染情况 ,从中随机选择 2 0例 ,采用免疫组化法检测单独的HPV 16感染及c erbB 2、p5 3和Rb表达。结果 :PCR检测食管癌组织HPV 16 / 18的感染率为 31 1% (2 8/ 90 ) ;免疫组化法检测食管癌组织中单独的HPV 16的感染率为 5 5 % (11/ 2 0 )。食管癌组织表达c erbB 2、Rb和p5 3和Rb的阳性率分别为 75 % (15 / 2 0 )、6 5 % (13/ 2 0 )和 80 % (16 / 2 0 )。HPV 16感染与c erbB 2、Rb和p5 3表达之间未见相关性。HPV 16感染以及c erbB 2、p5 3和Rb表达与淋巴结转移之间差异无显著性 (P >0 0 5 )。p5 3表达与食管癌组织学分级之间差异有显著性 (P <0 0 5 ) ,而HPV 16感染以及Rb和c erbB 2表达与食管癌组织学分级之间差异无显著性 (P >0 0 5 )。结论 :食管癌组织中HPV 16 / 18感染与c erbB 2、p5 3和Rb表达之间的关系是复杂的 ,尚有待于进一步研究。Objective HPV 16/18,c erbB 2,p53,Rb were examined in order to investigate their expression and significance in the oncogenesis of esophageal squamous carcinoma.Methods Infection of HPV 16/18 of the population in Chaoshan was detected by polymerase chain reaction(PCR);especially the infection of HPV 16 in addition to the expression of c erbB 2,p53,Rb was detected.Results The positive rate of HPV 16/18 was 31 1%(28/90)by PCR;the infectious rate of HPV 16 and the positive rate of c erbB 2,p53 and Rb were 55%(11/20),75%(15/20),65%(13/20),80%(16/20) by immunohistochemistry respectively.The relationship between p53 and the histological grade of esophageal carcinoma was significant.But no significant corelation was found about that the infection of HPV 16 and expression of p53,Rb and c erbB 2,that the relation of HPV 16,c erbB 2,Rb with the metastasis of the lymph nodes as well as the infection of HPV 16 and the expression of c erbB 2,Rb with the histological grade of esophageal carcinoma by statistic analysis ( P >0 05).Conclusions The intricate relation that the infection of HPV 16/18 and the expression of p53,Rb and c erbB 2 in the tissue of esophageal carcinoma are to be investigated.

关 键 词：食管鳞癌 HPV-16/18感染 C-ERBB-2 P53 RB 表达 

分 类 号：R735.1[医药卫生—肿瘤]