云南马铃薯青枯病菌的PCR检测  

SPECIFIC DETECTION OF RALSTONIA SOLANACEARUM IN POTATO BY PCR

作　　者：魏兰芳[1] 姬广海[2] 张世光[2] 

机构地区：[1]云南农业大学资源与环境学院,云南昆明650201 [2]云南农业大学植保学院,云南昆明650201

出　　处：《西南农业大学学报（自然科学版）》2002年第1期72-74,共3页Journal of Southwest Agricultural University

摘　　要：利用针对马铃薯青枯病菌hrp基因族DNA序列设计合成特异性寡核苷酸引物 (引物 1:5’GAAGAGGAACGACG GAAAGC - 3’和引物 2 :5’CGAACAGCCCACAGACAAGA - 3’) ,进行马铃薯青枯病菌PCR特异性扩增试验。该试验合成的引物能从马铃薯青枯病菌总基因组DNA和细菌纯培养 ,以及人工接种和自然发病的马铃薯块茎中特异性扩增青枯病菌hrp基因区段 1993bp的分子片段。该试验结果为马铃薯青枯病菌的鉴定、检测及病害流行学研究提供了新的技术和方法。A sensitive and specific assay based on a PCR protocol was develop for the detection of Ralstonia solanacearum , the pathogen for potato bacterial blight. Oligonucleotide specific primers (P1: 5'GAAGAGGAACGACGGAAAGC-3' and P2:5'CGAACAGCCCACAGACAAGA-3') were selected within the hrp gene region. Specific amplification of hrp fragments (1993 bp) was obtained for all the R. solanacearum strains studied. No amplification was observed for a wide range of other bacterial species. When applied to either pure culture or infected potato tubers, PCR allowed detection as low as 10 5 cfu/mL.

关 键 词：马铃薯 青枯病 诊断 hrp基因片段 云南马铃薯 PCR检测 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]