蛋白激酶C抑制剂Staurosporine与脑膜瘤细胞增生的相互关系  

The regulatory effects of protein kinase C in the proliferation of cultured human low-passage meningioma cel1s

作　　者：雷霆[1] 黄缤霖 李洛[1] 张龙[1] 薛德麟[1] 李龄[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经外科,武汉430030 [2]福建省泉州市第一人民医院神经外科,泉州362000

出　　处：《中国微侵袭神经外科杂志》2002年第1期38-40,共3页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：卫生部杰出青年科研基金(1997-164)

摘　　要：目的研究蛋白激酶C信号传导系统在人体外低代培养脑膜瘤细胞增生中的调节作用。初步探讨蛋白激酶C抑制剂对培养的脑膜瘤细胞增殖的影响及其作用机制。方法选取手术切除的新鲜脑膜瘤组织,常规早期传代细胞培养方法。观察蛋白激酶C抑制剂Staurosporine对细胞增殖的抑制率与基础磷脂酰肌醇(PI)转换率,比较分析上述两者间的相互关系。结果低代培养脑膜瘤细胞的增殖对蛋白激酶C抑制剂Staurosporine表现出剂量依赖性抑制效应。蛋白激酶C抑制剂对细胞增殖的抑制率与基础PI转换率之间存在正相关,r=0.58,P<0.01。结论蛋白激酶C信号传导系统介入人体外低代培养脑膜瘤细胞增生的调节。Objective To evaluate the potential role of the protein kinase C(PKC)-ediatedsignal transduction pathways in growth regu1ation,and to investigate the effects and the possible proliferation mechanism of low-passage human meningioma cells in vitro.Method Freshly resected meningiomas that obtained from the operation were placed into cell cultures.The numbers of the cultured meningioma cells were counted to evaluate the PKC inhibitor staurosporine on proliferation of menin-gioma cells.The basal phosphatidylinositol(PI)turnover rate and the inhibitory rate of starosporine were detected on the pro-liferation of the meningioma cells.Result The proliferation of the low-passagehuman meningioma cel1s was inhibited by stau-rosporine in a dose-dependentmanner.The inhibitory rate of staurosporine and the basal PI turnover rate assumed positive correlation,r=0.58,P<0.01.Conclusion PKC-mediatedsignal pathway is involved in the proliferation of the low-passagehu-man meningioma cells.

关 键 词：蛋白激酶C抑制剂Staurosporine 脑膜瘤 细胞增殖 蛋白激酶C 基础PI转换率 

分 类 号：R739.45[医药卫生—肿瘤]