c-myc反义基因对人高转移肺癌细胞增殖和黏附活性的抑制作用  

Inhibitive Effects of c-myc Antisense Oligodeoxynucleotides (ASODNS) on Proliferation and Adhesion on PG Cells

作　　者：李兆忠[1] 张玲[1] 王芸[1] 毛海婷[1] 温培娥[1] 李晓冰[1] 

机构地区：[1]山东省医学科学院基础医学研究所,济南250062

出　　处：《中国肿瘤生物治疗杂志》2002年第1期19-22,共4页Chinese Journal of Cancer Biotherapy

基　　金：山东省自然科学基金 (Y99C0 6 )

摘　　要：目的 :观察c myc反义基因抑制人高转移性肺癌PG细胞增殖 ,下调侵袭黏附性的作用和机制。方法 :合成c myc反义寡核苷酸 ,经脂质体包裹 ,转导入c myc高表达的PG细胞中。RT PCR方法检测c mycmRNA表达水平的变化 ,流式细胞术检测c myc蛋白的表达。MTT法检测细胞增殖活性 ,黏附实验检测PG细胞的黏附性。结果 :c myc反义基因 (>0 .6 2 5μmol/L)对PG细胞c mycmRNA、蛋白表达和细胞增殖活性具有明显的抑制作用 ,其处理PG细胞 72h后 ,2 0～ 80min不同时间的细胞黏附百分率 ,从 5 0 .0 %,81.2 7%和 90 .0 %分别显著下降到 31.5 %,37.5 %和 30 .0 %(P <0 .0 5 ) ,下降呈时间依赖效应。结论 :c myc反义基因抑制PG细胞c mycmRNA和蛋白表达水平 ,同时下调增殖活性和侵袭黏附性。Objective: To observe the role and probable mechanisms of c-myc ASODNS on PG cells proliferation and the level of ahesion downregulated. Methods: c-myc antisense phosphorothioate oligonucleotides were synthesized and purified and then lipofectin reagent (Lf) interacted spontaneously with ASODNS to form a lipid-DNA complex(ASODNS-Lf).Finally,ASODNS-Lf was used to successfully transfer ASODNS into PG cells. c-myc mRNA was examined by RT-PCR. MTT assay was used to explore the effects of c-myc ASODNS on PG cells proliferation. PG cells ahesion was examined by LN. Results: 1. After PG cells were treated with ASODNS-Lf (> 0.625 μmol/L), there was a marked expression reduction of c-myc mRNA and oncoprotein as well as cells proliferation. 2.PG cells adhesive ratio to laminin substrate after adhesion 20～80 min decreased from 50.0%,81.27% and 90.0% to 31.5%,37.5% and 30.0%(P<0.05 ).Conclusions: c-myc ASODNS-Lf can inhibit the expression of c-myc mRNA and protein and down-regulates PG cells growth and the level of cell adhesion .

关 键 词：细胞增殖 细胞黏附性 基因治疗 c-myc反义基因 人高转移肺癌细胞 抑制作用 

分 类 号：R734.2[医药卫生—肿瘤] R730.5[医药卫生—临床医学]