东亚钳蝎毒抗癌多肽对人肿瘤细胞系和动物移植瘤的作用  

Effect of antineoplastic polypeptide from Buthus martensii venom on human tumor cell lines and animal transplanting tumors

作　　者：魏玲[1] 董伟华[2] 孔天翰[2] 

机构地区：[1]山东省肿瘤医院基础研究中心,山东济南250117 [2]河南医科大学生物毒素中心,河南郑州450052

出　　处：《中国癌症杂志》2002年第2期97-101,共5页China Oncology

基　　金：河南省计委重大科技攻关项目 (96 5 9)

摘　　要：目的 :研究东亚钳蝎毒抗癌多肽 (APBMV)对人早幼粒白血病细胞系HL 6 0和人肝癌细胞系SMMC 772 1及对小鼠肝癌H2 2 移植瘤的作用。方法 :体外应用MTT法、生长抑制实验和集落形成实验 ,观察APBMV对HL 6 0、SMMC 772 1及H2 2 瘤细胞的作用 ;体内实验采用小鼠H2 2 实体瘤模型 ,检测肿瘤生长抑制率 (IR)、白细胞计数(WBC)和脾脏指数 (SI) ,观察APBMV对H2 2 带瘤小鼠的影响。结果 :APBMV对HL 6 0和SMMC 772 1细胞均具较强的毒性作用 ,其IC50 值分别为 10 .74 μg ml和 11.33μg ml;APBMV可显著抑制两种细胞的生长 ,剂量效应关系明显 ,HL 6 0和SMMC 772 1细胞 2 4h、4 8h和 96h的IC50 值分别为 19.4 1μg ml、9.90 μg ml、11.4 1μg ml和l5 .87μg ml、13.0 5μg ml、8.70 μg ml;APBMV浓度 >8μg ml时 ,可明显抑制SMMC 772 1细胞的集落形成 (P <0 .0 1) ,IC50 为 10 .83μg ml。H2 2 细胞经APBMV处理后 ,代谢MTT的能力明显低于对照组 ,但剂量效应关系不明显 (P >0 .0 5 )。APBMV能显著抑制H2 2 带瘤小鼠的肿瘤生长 ,IR最高达 4 0 .30 % (P <0 .0 1) ,小鼠WBC和SI无明显变化或略高于对照组 ,而 5 Fu组小鼠WBC和SI均明显下降 (P <0 .0 1)。结论 :APBMV是东亚钳蝎蝎毒中有效低毒的抗肿瘤成分 ,对HL 6 0、SMMCPurpose:To investigate the effect of antineoplastic polypeptide from Buthus martensii venom (APBMV) on the cultured human promyelocytic leukemia cells HL 60 and hepatoma cell line SMMC 7721 and hepatoma H 22 bearing mice.Methods:MTT colorimetric method, growth inhibiting test and colony formation assay were used in the in vitro test. H 22 bearing mice were applied in the in vivo experiment. Through measuring tumor growth inhibitory rate(IR),white blood cell (WBC) number and spleen index (SI) ,we explored the influence of APBMV on H 22 bearing mice.Results:APBMV possessed stronger cytotoxicity on HL 60 cells and SMMC 7721 cells, and IC 50 was 10.74 μg/ml and 11.33 μg/ml , respectively. APBMV could dramatically inhibit their growths. There were obvious dosage response correlations. The IC 50 of HL 60 and SMMC 7721 at 24h, 48h and 96h were 19.41 μg/ml, 9.90 μg/ml, 11.41 μg/ml and 15.87 μg/ml, 13.05 μg/ml, 8.70 μg/ml, respectively. When the concentration of APBMV exceeded 8 μg/ml, the colony formation rate of SMMC 7721 cells decreased dramatically ( P <0.01).Dosage effect relationship was evident, and IC 50 was 10.83 μg/ml . The ability to metabolize MTT of H 22 cells was lower than control distinctively after the cells were treated by APBMV, whereas dose response relationship was not clear(P>0.05).Tumor growth of H 22 bearing mice was markedly inhibited by APBMV,the growth inhibiting rate was reached 40.30% ( P <0.01). After treatment by APBMV , white blood cell(WBC) number and spleen index of H 22 bearing mice were not changed markedly or were slightly higher than control group. In 5 fluorouracil treated group, WBC number and spleen index of H 22 bearing mice were all reduced markedly( P <0.01). Conclusions:APBMV was one kind of effective and low toxicity antineoplastic components in the venom of Buthus Martensii Scorpion. It possesses dramatic inhibitory effect on HL 60 and SMMC 7721 cells and neoplasms of H

关 键 词：东亚钳蝎毒 抗癌多肽 人肿瘤细胞系 动物移植瘤 APBMV 

分 类 号：R73－35[医药卫生—肿瘤] R996.3[医药卫生—临床医学]