食管鳞癌nm23-H_1蛋白表达及DNA倍体与淋巴结转移关系  

The relation between nm23-H_1 protein expression or DNA ploidy and lymph node metastasis of esophageal squamous cell carcinoma.

作　　者：王兴武[1] 国前[1] 魏玲[1] 宋现让[1] 孙菊杰[1] 田国栋[1] 

机构地区：[1]山东省肿瘤防治研究院基础研究中心,山东济南250117

出　　处：《中国癌症杂志》2002年第2期133-136,共4页China Oncology

摘　　要：目的 :研究食管鳞癌nm2 3 H1 蛋白表达及DNA倍体状态与区域淋巴结转移的关系。方法 :应用流式细胞仪检测食管癌患者新鲜手术标本包括食管癌组织 (癌 )、食管癌旁粘膜 (癌旁 )、食管正常粘膜 (切缘 )及食管癌区域淋巴结 (淋巴结 )中nm2 3 H1 蛋白表达水平及DNA倍体状态。结果 :无论有、无淋巴结转移 ,nm2 3 H1 蛋白表达在癌与癌旁、癌与切缘之间差异有显著性 (P <0 .0 5 ) ,癌与淋巴结之间差异无显著性 (P >0 .0 5 ) ;DNA指数 (DI)在癌组织与癌旁、切缘、淋巴结之间比较差异均有显著性 (P <0 .0 5 ) ,癌旁、切缘、淋巴结之间DI差异无显著性 (P >0 .0 5 ) ;nm2 3 H1 蛋白表达在有、无淋巴结转移两组对应组织间比较差异均有显著性 (P <0 .0 5 ) ;两组对应组织比较DI差异无显著性 (P >0 .0 5 ) ;DI在不同病理分级间差异有显著性 (P <0 .0 5 ) ,DI及nm2 3 H1 蛋白表达在性别、肿瘤部位、病理类型、浸润深度间差异无显著性 (P >0 .0 5 )。结论 :nm2 3 H1 蛋白高表达对食管癌转移有一定的抑制作用 。Purpose:To study the relation between regional lymph node metastasis and nm23 H 1 protein expression and DNA ploidy of esophageal squamous cell carcinoma patients. Methods:Flow cytometry was used to analyze nm23 H 1 protein and DNA ploidy in surgical specimens from the patients with esophageal carcinoma. The object tissues included: the primary tumor tissue, the pericancerous mucosa, the incisional margin and the regional lymph node. Results:There were obvious differences between nm23 H 1 protein expression in lymph node metastasis positive and negative groups, primary tumor tissue and pericancerous mucosa, cancer tissue and the incisal margin( P <0.05).This phenomena was not seen between cancer tissue and lymph node( P >0.05).The differences of DI were distinct between cancer tissue and the pericancerous mucosa, cancer tissue and the incisal margin, cancer tissue and the lymph node( P <0.05),the phenomena were not seen among pericancerous mucosa and incisal margin and lymph node( P >0.05).In corresponding tissues of lymph node metastasis positive and negative groups, nm23 H 1 protein expression was different( P <0.05), but DI was similar( P >0.05). As to DI, it was different only among pathological grades( P <0.05).There was no difference between DI or nm23 H 1 protein expression and sex, tumor position, pathological type, infiltrating depth( P >0.05). Conclusions:nm23 H 1 protein higher expression can produce certain inhibitory effect on metastasis of esophageal carcinoma. Combining DNA ploidy detection can help to understand the biological behavious and deduce the prognosis of esophageal carcinoma.

关 键 词：食管鳞癌 NM23-H1蛋白 DNA倍体 淋巴结转移 流式细胞术 

分 类 号：R735.1[医药卫生—肿瘤] R73－37[医药卫生—临床医学]