胃癌细胞上调FasL表达对胃癌细胞凋亡的影响  

Effect of Up-expression of Fas Ligand on the Apoptosis of Gastric Carcinoma

作　　者：黄群[1] 高晓燕[2] 陈江[1] 纪礽斌[1] 胡伟[1] 刘国焰[1] 吕农华[1] 王崇文[1] 

机构地区：[1]江西医学院第一附属医院消化研究所,江西南昌330006 [2]江西省吉安市中心人民医院消化科,江西吉安343000

出　　处：《江西医学院学报》2002年第2期27-29,共3页Acta Academiae Medicinae Jiangxi

摘　　要：目的 :观察胃癌FasL蛋白表达及其对胃癌细胞凋亡的影响。方法 :采用免疫组化染色对 5 0例手术切除的胃腺癌组织中FasL蛋白进行观察 ;采用脱氧核糖核酸末端转移酶介导的缺口末端标记 (TUNEL)技术 ,对其中 4 0例胃癌组织中胃癌细胞的凋亡情况进行原位观察。结果 :5 0例胃腺癌组织均表达FasL蛋白 ,阳性率为 10 0 % ,明显高于正常胃粘膜的 4 5 % (P <0 .0 1) ,胃腺癌组织 78%中、强表达 ,正常胃粘膜均为弱表达。随着胃癌细胞FasL表达水平的增加 ,胃癌细胞的凋亡指数逐渐下降 ,各组间差异有显著性 (P <0 .0 1) ,胃癌细胞的凋亡水平与其FasL表达呈负相关。结论 :胃腺癌细胞可能通过上调表达FasL ,间接地使胃癌细胞的凋亡下调而保护了自己 ,这可能是胃癌免疫逃逸的机制之一。Objective:To investigate the effect of up-regulation of FasL ligand protein on the apoptosis of gastric carcinoma(GC). Methods: expression of FasL protein in 50 resected GC and apoptosis of cancer cells were detected with immunohistochemistry and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL). Results: The expressions of FasL were detected in all of 50 resected GC with positive rate of 100%, which was significantly higher than that (45%) in control normal gastric epithelia( P <0.01).In contrast, the FasL expression was mediated or extensive in 78% of GC,( P <0.01).As the FasL expression became more extensive, the apoptosis index of GC cells decreased stepwisedly. Conclusion:Human GC may down regulate its apoptosis through up regulating its expression of FasL.Therefore,the cancer may protect itself from immune surveillence.

关 键 词：FASL 胃腺癌 细胞凋亡 免疫逃逸 免疫组织化学染色 

分 类 号：R735.2[医药卫生—肿瘤] R730.3[医药卫生—临床医学]