纤维粘连蛋白在牙髓修复中作用的免疫组织化学研究  

Roles of Fibronectin in Reparative Dentinogenesis:An Immunohistochemical Study

作　　者：陈智[1] 樊明文[1] 吕平 边专[1] 张旗[1] 

机构地区：[1]武汉大学口腔医学院,430070

出　　处：《华西口腔医学杂志》2002年第2期104-106,共3页West China Journal of Stomatology

基　　金：国家自然科学基金 (编号 3 983 0 43 0 ;湖北省自然科学基金资助项目 (编号 98J0 96)

摘　　要：目的 :研究纤维粘连蛋白在牙髓修复中的免疫定位和分布特征。方法 :在大鼠第一磨牙制备单面洞 ,分别观察 3d、15d和 30d后修复性牙本质形成情况。免疫组织化学技术检测纤维粘连蛋白的免疫反应。结果 :术后 3d ,修复性牙本质尚未形成。牙髓内成牙本质细胞呈弱阳性染色 ,前期牙本质也为弱阳性。术后 15d ,可见修复性牙本质形成 ,成牙本质细胞样细胞和牙髓成纤维细胞免疫染色呈阳性。术后 30d ,已形成的修复性牙本质内纤维粘连蛋白染色为强阳性 ,特别集中于修复性牙本质与牙髓的交界面 ,成牙本质细胞样细胞染色呈强阳性。结论 :纤维粘连蛋白可能在修复性牙本质形成中起重要调节作用。Objective: The aim of this study was to investigate roles of fibronectin in dental pulp injury and repair. Methods: The cavity was prepared in the mesial surface of the first molars of Wistar rats. The rats were sacrificed in 3, 15, and 30 days post_operation. After histological process, the paraffin sections were reacted with monoclonal antibodies against rat fibronectin using SABC method.Results: Immunohistochemical stain demonstrated that the positive staining of fibronectin presented in different stages of dental pulp repair. In the group of 3 days, the weak staining was located in odontoblasts. After 15 days, odontoblast_like cells and dental pulp cells were stained positively. 30 days after operation, the stronger staining was observed in odontoblast_like cells and pulp fibroblasts. Positive staining was seen in reparative dentin, particularly along the interface of reparative dentin and dental pulp.Conclusion: These results demonstrated that fibronectin might play a role of mediation during the reparative dentinogenesis.\;

关 键 词：修复性牙本质 纤维粘连蛋白 牙髓 免疫组织化学 修复 

分 类 号：R780.2[医药卫生—口腔医学]