单细胞凝胶电泳技术在胚胎毒性实验中的应用  

The application of single cell gel electrophoresis in experiment exmaing embryonal toxicity

作　　者：李树蕾[1] 周莉[1] 陈辉[2] 黄可欣[1] 聂毓秀[1] 鄢励 刘颖丽 

机构地区：[1]吉林大学基础医学院组织胚胎学教研室,吉林长春130021 [2]吉林卫生学校组织胚胎学教研室 [3]吉林省妇幼保健院 [4]吉林省长春市医学高等专科学校

出　　处：《吉林大学学报（医学版）》2002年第2期124-126,共3页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题 [2 9890 2 82 - 3(2 )

摘　　要：目的 :探讨和分析单细胞凝胶电泳技术 ( SCGE)的影响因素和实验中应该注意的环节。方法 :通过对实验方法和操作技术的总结 ,对 Singh提出的碱性 SCGE进行适当的补充。结果 :完善了 SCGE的操作 ,获得了典型的彗星图像。结论 :在实验中避免非特异性因素的影响 ,注意实验操作细节 ,能够增加Objective:To investigated and analyzed the factors influencing on the results and the links attended. Methods:SCGE was modified properly during the test based on the basic SCGE advanced by Singh 1988 after summarizing experiment method and skill. Results:The operation of SCGE was perfected and the typical comet star was obtained. Conclusion:Specificity and sensitivity of SCGE would be enhanced if the effects of nonspecific factors were avoided and more attention was paid carefully during the assay.

关 键 词：单细胞凝胶电泳 胚胎毒性 应用 动物实验 彗星图像 SCGE 

分 类 号：R446.6[医药卫生—诊断学]