木本果树遗传转化研究进展  

Advances in Research on Genetic Transformation in Woody Fruit Crops

作　　者：曾黎辉[1] 吕柳新[1] 

机构地区：[1]福建农林大学园艺系,福建福州350002

出　　处：《果树学报》2002年第3期191-198,共8页Journal of Fruit Science

摘　　要：从遗传转化方法和影响遗传转化的因素两方面综述了木本果树遗传转化技术近年来的进展。木本果树遗传转化方法主要有农杆菌介导法和基因枪法;影响转化的因素主要从受体与再生系统、选择标记基因与转化细胞的筛选、培养方法与培养条件等方面进行论述。文章还归纳了导入目的基因并获得再生植株的木本果树种类,并对木本果树基因工程进展及展望进行了讨论。This article presents an overview of the progress in genetic transformation of woody fruit crops,particular attention is given to the methods of transformation and the factors affecting gene transfer.Agrobacterium-mediated and particle biolistics are the main methods used in genetic transformation of fruit trees.The factors affecting gene transfer are discussed from several aspects,such as plant targets and its regeneration system,marker gene and the selection of transformed cells and the conditions of culture.Papers reporting the introduction of target genes into woody fruit crops have grouped into one table.The article also discusses the development of genetic engineering of woody fruit crops.

关 键 词：木本果树 遗传转化 基因工程 农杆菌介导 基因枪法 受体 再生系统 再生植株 

分 类 号：S66[农业科学—果树学] Q785[农业科学—园艺学]