抗虫基因(CpTI)辣椒转化的初步研究  

Introduction of Insect-Resistant Gene CpTI in Pepper

作　　者：王朋[1] 王关林[2] 方宏筠[2] 

机构地区：[1]中国科学院沈阳应用生态研究所,辽宁沈阳110015 [2]辽宁师范大学生物工程研究所,辽宁大连116029

出　　处：《沈阳农业大学学报》2002年第1期30-32,共3页Journal of Shenyang Agricultural University

基　　金：辽宁省"十五"科技攻关项目 (2001215003)

摘　　要：以辣椒叶片为外植体 ,通过比较基因型、培养基成分等因素对不定芽分化的影响 ,建立了具有较高分化频率的再生系统。用农杆菌介导把豇豆胰蛋白酶抑制剂(CpTI)基因导入辣椒 ,在筛选培养基上获得抗性植株 ,经Southern斑点杂交检测 。A shoot regeneration system of high frequency in pepper (Capsicum annuum L.) was established with leaf as explant, in consideration of the effect of genotypes, medium ingredients for adventitious shoot differentiation. The CpTI gene was transformed into the cells of pepper via Agrobacterium (pRICpTI) and resistant plants were obtained in the screening medium. The result of Southern Dot analysis verified preliminarily that exogenous gene had been integrated to the genome of Capsicum.

关 键 词：辣椒 快速繁殖 基因转化 抗虫基因 CPTI 农杆菌介导 抗性植株 再生体系 

分 类 号：S641.3[农业科学—蔬菜学]