大黄鱼生长激素基因的分离及序列测定  

The Sequence Analysis of Growth Hormone Gene from pseudosciaena crocea

作　　者：江树勋[1] 马鸿媚[1] 邓文汉[2] 饶平凡[2] 

机构地区：[1]福州市海洋生物工程研究开发中心,福州350026 [2]福州大学生物工程研究所,福州350002

出　　处：《生物工程进展》2002年第2期88-90,共3页Progress in Biotechnology

基　　金：福建省科委资助项目

摘　　要：本文从大黄鱼 (Pseucdosiaenacrocea)脑下垂体中提取得到总RNA ,根据近源鱼种生长激素(GH)基因保守序列设计了上游和下游引物 ,通过反转录和PCR扩增得到大黄鱼生长激素cDNA片断 ,并进行序列分析。所得到的序列与近源鱼种生长激素基因序列有较高的同源性 ,断定为大黄鱼生长激素基因序列 。This article studies on the growth hormone(GH)gene sequence of Pseudosciaena crocea.Total RNA is extracted from the pituity of Pseudosciaena crocea and primers are designed according to its congenetic fish species'GH gene.After RT-PCR,the cDNA piece is gotten and sequenced.Compared to those congenetic species,a high homologus was found and it is concluded to be the GH gene sequence of Pseudosciaena crocea,This study sets the foundation of further investigating bioactivity and application of this gene.

关 键 词：大黄鱼 生长激素基因 分离 序列测定 反转录PCR 

分 类 号：S965.322[农业科学—水产养殖] S961.6[农业科学—水产科学]