三株肝癌单克隆抗体相应抗原在肝癌细胞中的表达与分布  

THE EXPRESSION AND DISTRIBUTION OF THREE HUMAN HEPATOMA CARCINOMA mAbs'ANTIGENSIN HEPATOMA CELL LINES

作　　者：黄宝成[1] 陈志南[1] 夏永娟[2] 邢金良[1] 姜绍谆[3] 

机构地区：[1]第四军医大学细胞工程研究中心,西安710032 [2]第四军医大学组织学与胚胎学教研室,西安710032 [3]第四军医大学微生物学教研室,西安710032

出　　处：《中国组织化学与细胞化学杂志》2000年第3期316-318,共3页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家863资助课题!(No. 86 3- 10 2 - 0 9- 0 1)

摘　　要：应用免疫细胞化学方法、图像分析技术和免疫电镜技术 ,对 3株肝癌 m Abs A10 ,E5和 F11在肝癌细胞株中的表达情况及分布特点进行了研究。结果显示 :3株单抗抗原在肝癌细胞株 HHCC中均有较高表达 ;单抗 A10 ,F11相应抗原主要分布在肝癌细胞的胞膜和胞浆中 ,而在胞核上无明显分布。单抗 E5相应抗原除分布在胞膜和胞浆外 ,在细胞核上也有明显浓聚。本实验明确了 3株肝癌单抗相应抗原在肝癌细胞中的分布特点 ,对于深入分析抗原的本质有重要参考价值。In order to investigate the expression and distribution characteristics of three human hepatoma m Abs' ( A1 0 ,E5,F1 1 ) antigen in hepatoma cell lines,the immunocytochemical method,image analysis and immunoelectron microscopic technique were used.The results were as follows:( 1 ) we found a hepatoma cell line HHCC which had a relatively high expression of three m Abs'antigens. ( 2 ) m Abs A1 0 and F1 1 's antigen was localized in cytoplasm and or cell membrane,whereas m Ab E5'santigen had a higher concentration in nucleus than in other partof hepatoma cell.These results help us to choose a suitable cell line that we will use to constructa c DNA expression library screened by the m Abs,and also give us some suggestions of the nature of the m Abs'antigens.

关 键 词：肝癌 单克隆抗体 抗原 表达 分布 免疫细胞化学 图像分析 免疫电镜 小鼠 

分 类 号：R735.7[医药卫生—肿瘤]